Regulation of lutein biosynthesis and prolamellar body formation in Arabidopsis

Functional Plant Biology : FPB
Abby J CuttrissB Pogson

Abstract

Carotenoids are critical for photosynthetic function in chloroplasts, and are essential for the formation of the prolamellar body in the etioplasts of dark-grown (etiolated) seedlings. They are also precursors for plant hormones in both types of plastids. Lutein is one of the most abundant carotenoids found in both plastids. In this study we examine the regulation of lutein biosynthesis and investigate the effect of perturbing carotenoid biosynthesis on the formation of the lattice-like membranous structure of etioplasts, the prolamellar body (PLB). Analysis of mRNA abundance in wildtype and lutein-deficient mutants, lut2 and ccr2, in response to light transitions and herbicide treatments demonstrated that the mRNA abundance of the carotenoid isomerase (CRTISO) and epsilon-cyclase (ϵLCY) can be rate limiting steps in lutein biosynthesis. We show that accumulation of tetra-cis-lycopene and all-trans-lycopene correlates with the abundance of mRNA of several carotenoid biosynthetic genes. Herbicide treatments that inhibit carotenoid biosynthetic enzymes in wildtype and ccr2 etiolated seedlings were used to demonstrate that the loss of the PLB in ccr2 mutants is a result of perturbations in carotenoid accumulation, not indirect sec...Continue Reading

References

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May 4, 2006·Annual Review of Plant Biology·Dean DellaPenna, Barry J Pogson

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Citations

Aug 21, 2008·Journal of Integrative Plant Biology·Shan Lu, Li Li
Oct 2, 2009·Plant Signaling & Behavior·Christopher Ian CazzonelliBarry J Pogson
Jun 3, 2011·Plant & Cell Physiology·Kim Gabriele BeiselShizue Matsubara
Jul 15, 2009·The Plant Journal : for Cell and Molecular Biology·Antía Rodríguez-VillalónManuel Rodríguez-Concepción
Oct 15, 2009·Plant Signaling & Behavior·Antía Rodríguez-VillalónManuel Rodríguez-Concepción
May 15, 2012·The Arabidopsis Book·M Águila Ruiz-Sola, Manuel Rodríguez-Concepción
Nov 1, 2011·Functional Plant Biology : FPB·Christopher I Cazzonelli
Feb 1, 2020·ELife·Christopher I CazzonelliBarry J Pogson
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Michał BykowskiŁucja Kowalewska
Jun 30, 2016·Molecular Plant·Sekhar KambakamSteve Rodermel
May 18, 2021·Frontiers in Plant Science·Katalin Solymosi, Beata Mysliwa-Kurdziel
Dec 3, 2009·Molecular Plant·Christopher I CazzonelliBarry J Pogson

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Methods Mentioned

BETA
PCR
transgenic
electron microscopy

Software Mentioned

CRTISO

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