PMID: 7540043May 29, 1995Paper

Regulation of lysosomal and ubiquitin degradative pathways in differentiating human intestinal Caco-2 cells

Biochimica Et Biophysica Acta
Y ZhangN M Dahms

Abstract

The expression of various components of the lysosomal and ubiquitin-dependent degradative pathways was characterized in an in vitro model of differentiating enterocytes, the human colon adenocarcinoma Caco-2 cell line. The activities of the cell-associated lysosomal enzymes alpha-D-mannosidase, beta-hexosaminidase, beta-glucuronidase, and beta-galactosidase increased approximately 2- to 4-fold as differentiation proceeded. In contrast, the protein levels of the two mannose 6-phosphate receptors (MPRs), the insulin-like growth factor II/cation-independent MPR (IGF-II/CI-MPR) and the cation-dependent MPR (CD-MPR), did not change significantly during Caco-2 differentiation. In addition, quantitative Western blot analyses revealed that on a molar basis the CD-MPR is 3.5 times more abundant than the IGF-II/CI-MPR in Caco-2 cells. Since only limited secretion of lysosomal enzymes was observed throughout differentiation, the level of expression of the MPRs was sufficient to target the increased levels of lysosomal enzymes to the lysosome. Unlike the expression of lysosomal enzymes, Western blot analysis demonstrated an approximately 40% and approximately 30% decrease, respectively, in the steady-state levels of free and conjugated ubi...Continue Reading

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Citations

Jun 17, 2010·Genes & Development·Nan Gao, Klaus H Kaestner
Dec 6, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·U ThormannG Imanidis
Dec 17, 2015·Journal of Animal Physiology and Animal Nutrition·H ZhangS Bai
Dec 26, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·Debra A WickNancy M Dahms

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