PMID: 8938724Jan 1, 1996Paper

Regulation of M-type potassium channels in mammalian sympathetic neurons: action of intracellular calcium on single channel currents

Neuropharmacology
A A Selyanko, D A Brown

Abstract

Currents through single M-type potassium channels were recorded in membrane patches excised from rat superior cervical sympathetic neurons. Application of Ca+ to the internal face of inside-out patches produce two forms of M-channel inhibition: a slow, all-or-nothing suppression of activity; and a fast block associated with a concentration-dependent shortening of open times compatible with open-channel block. Both forms of block were enhanced by patch depolarization. Neither was replicated or affected by Mg2+, and both could be recorded in the absence of intracellular ATP, implying that they did not involve phosphorylation. Since the block was reversible in the absence of ATP and since alkaline phosphatase did not reduce channel activity, block was unlikely to have resulted from dephosphorylation. In cell-attached patch recording, M-channel activity increased during exposure of the cell to Ca2(+)-free solution and was rapidly reduced on applying 2mM Ca2+ to the extra-patch solution. This suggests that M-channel activity in these neurons may be tonically regulated by variations in resting intracellular [Ca2+].

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L BernheimB Hille
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J BeechB Hille
Apr 1, 1991·Neuron·N V MarrionP R Adams
Jan 1, 1996·Trends in Biochemical Sciences·M Ikura

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Citations

Jun 18, 2003·The Journal of General Physiology·Nikita Gamper, Mark S Shapiro
May 20, 2005·Molecular Biology of the Cell·Nikita GamperMark S Shapiro
Mar 15, 2008·The Journal of Physiology·Manjot BalMark S Shapiro
Aug 1, 2006·Biochemical and Biophysical Research Communications·Ching-Chyuan SuMin-Jon Lin
Apr 16, 2008·Expert Opinion on Therapeutic Targets·Valentin K Gribkoff
Jul 29, 1999·Biophysical Journal·A A Selyanko, D A Brown
Apr 20, 2007·Biochimica Et Biophysica Acta·Kirstine CalloeNanna Koschmieder Jorgensen
Jan 14, 2018·The Journal of Physiology·Arielle L BakerAllan T Gulledge
May 2, 2008·American Journal of Physiology. Cell Physiology·Boyi LiuHailin Zhang
Dec 9, 1998·The American Journal of Physiology·J C Gomora, J J Enyeart

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