PMID: 1202246Dec 1, 1975Paper

Regulation of macromolecular synthesis in reovirus-infected L-929 cells I. Effect of L-histidinol

Journal of Virology
R C Warrington, N Wratten

Abstract

The histidine analogue L-histidinol, reported by Vaughan and Hansen (1973) to establish a potent, readily reversible inhibition of eukaryotic protein synthesis in vivo, was used to investigate the regulation of macromolecular synthesis in reovirus-infected L-929 cells. The addition of L-histidinol to normal L cells led to a total inhibition of protein synthesis. The inhibition appeared to be a consequence neither of isotope dilution resulting from elevated endogenous amino acids nor of an inability of treated cells to accumulate exogenous amino acids. Addition of L-histidine to histidinol-arrested cells resulted in a complete recovery of protein synthesis. Similarly, protein synthesis in reovirus-infected L cells examined 17 h postinfection (31 C) was totally inhibited by histidinol treatment and was readily reversed by the addition of histidine. Reovirus-infected cells treated with histidinol had an essentially unaltered capacity to synthesize reovirus single-stranded RNA relative to unperturbed cultures but a diminishing ability to maintain genome RNA synthesis. Addition of L-histidine to arrested cultures led to a complete recovery of genome RNA synthesis. The L-histidinol-mediated arrest of protein synthesis was both very e...Continue Reading

References

Aug 1, 1974·Journal of Virology·J Lucas-Lenard
Nov 1, 1972·Virology·H J ZweerinkT Matsuhisa
Dec 7, 1973·Biochimica Et Biophysica Acta·R C WarringtonA M Kapuler
Mar 14, 1972·Biochimica Et Biophysica Acta·R P PerryJ R Greenberg
Jul 1, 1970·Virology·H J Zweerink, W K Joklik
Feb 1, 1967·Journal of Virology·Y WatanabeA F Graham
Aug 28, 1968·Journal of Molecular Biology·Y WatanabeA F Graham
Jul 20, 1966·Biochemical and Biophysical Research Communications·H Kudo, A F Graham

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Sep 1, 1977·Virology·R C Warrington, N Wratten

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