Regulation of magnesium content during proliferation of mammary epithelial cells (HC-11)

Frontiers in Bioscience : a Journal and Virtual Library
Federica I WolfA Cittadini

Abstract

To study the role of Magnesium in the regulation of cell proliferation we characterized the proliferation behaviour of HC-11 mammary epithelial cells that were grown in media containing low to high Mg concentrations. Cells grown under control conditions (0.5 mM Mg in the medium) or in the presence of high (H) Mg (45 mM) displayed similar log-phases and reached confluence in 72h. In the presence of low (L) Mg (0.025 mM) the cells exhibited a reduced growth rate and did not reach confluence at 72h. Intra cellular total Mg increased from 12 to 36h of culture in all cells examined but returned to basal levels in those cells which reached confluence (i.e., control and H-Mg cells). Intra cellular Mg increased independent of mitosis-induced changes of volume and adenine nucleotides pools but correlated with an increased percentage of cells in the S phase and with total nucleic acid contents. These bell-shaped changes of intra cellular Mg were less evident in L-Mg cells, likely due to a combination of low Mg levels in the medium and decreased growth rate. Changes in membrane potential and pH were important factors that contributed to maintaining intra cellular Mg at physiologic levels in the face of increased or decreased availability ...Continue Reading

Citations

Nov 16, 2007·Nutrition and Cancer·Jeanette A M MaierFederica I Wolf
May 20, 2004·Science's STKE : Signal Transduction Knowledge Environment·Federica I Wolf
Jan 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Charles Vidair, Harry Rubin
Apr 25, 2013·Journal of the American Chemical Society·Zhen WangAmnon Kohen
Feb 11, 2009·Cancer Treatment Reviews·Federica I WolfJeanette A M Maier
Feb 27, 2016·Chemical Research in Toxicology·Valentina TrapaniFederica I Wolf
Feb 17, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Harry Rubin
Jun 8, 2012·Clinical Science·Federica I Wolf, Valentina Trapani
Dec 3, 2014·Biochemical and Biophysical Research Communications·Valentina TrapaniFederica I Wolf
Mar 7, 2014·Tissue Engineering. Part a·Tiejun QuXiaohua Liu
May 18, 2018·The FEBS Journal·Serge HardyMichel L Tremblay
Dec 1, 2007·Clinical Science·Federica I Wolf, Valentina Trapani
Dec 30, 2014·Physiological Reviews·Jeroen H F de BaaijRené J M Bindels
Mar 13, 2016·The Journal of Biological Chemistry·Elie KostantinMichel L Tremblay

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