Regulation of matrix metalloproteinases 2 and 9 activities by peroxynitrites in term placentas from type 2 diabetic patients

Reproductive Sciences
E CapobiancoA Jawerbaum

Abstract

Matrix metalloproteinases (MMPs) are proteolytic enzymes related to a proinflammatory environment in several diseases, including diabetes, which can be activated by reactive nitrogen species. This work aimed to determine MMP-2 and MMP-9 activities and nitration in term placentas from type 2 diabetic patients and verify the hypothesis that peroxynitrites are positive regulators of placental MMP-2 and MMP-9 activities. For this purpose, term placentas from healthy and type 2 diabetic patients were analyzed for MMP-2 and MMP-9 levels and activities, protein nitration, and nitration of MMP-2 and MMP-9. Villous explants were cultured in the presence of peroxynitrites for further evaluation of MMP-2 and MMP-9 activities. We found that MMP-2 and MMP-9 activities were increased in term placentas from diabetic patients. These changes were found even when MMP-2 protein concentrations were diminished and MMP-9 protein concentrations were not changed in the diabetic group. Increased protein nitration and specific nitration of MMP-2 and MMP-9 were found in term placentas from diabetic patients. Peroxynitrites were able to increase the activity of placental MMP-2 and MMP-9. Taken together, this study has shown for first time that peroxynitri...Continue Reading

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Citations

Oct 21, 2014·American Journal of Reproductive Immunology : AJRI·Ratana Lim, Martha Lappas
Sep 24, 2014·Reproductive Toxicology·Romina HigaAlicia Jawerbaum
Apr 6, 2017·Reproductive Biology and Endocrinology : RB&E·Joel Ramírez-EmilianoJessica K Órnelas-Vázquez
May 19, 2018·International Journal of Molecular Sciences·Rajaa AouacheFrancisco Miralles
May 10, 2013·American Journal of Physiology. Cell Physiology·Patricia A ClarkMark S Roberson
Nov 6, 2014·Hormone Molecular Biology and Clinical Investigation·Gabriela BergVeronica Miksztowicz

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Methods Mentioned

BETA
cesarean section
electrophoresis
Assay
enzyme-linked immunosorbent assay
immunoprecipitation

Software Mentioned

Image J
Prism
GraphPad

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