Regulation of Metabolism by an Ensemble of Different Ion Channel Types: Excitation-Secretion Coupling Mechanisms of Adipokinetic Hormone Producing Cells in Drosophila

Frontiers in Physiology
Rebecca J PerryErik C. Johnson

Abstract

Adipokinetic Hormone (AKH) is the primary insect hormone that mobilizes stored energy and is functional equivalent to mammalian glucagon. While most studies have focused on exploring the functional roles of AKH, relatively little is known about how AKH secretion is regulated. We assessed the AKH cell transcriptome and mined the data set for specific insight into the identities of different ion channels expressed in this cell lineage. We found reliable expression of multiple ion channel genes with multiple members for each ionic species. Specifically, we found significant signals for 39 of the either known or suspected ion channel genes within the Drosophila genome. We next performed a targeted RNAi screen aimed to identify the functional contribution of these different ion channels that may participate in excitation-secretion coupling in AKH producing cells (APCs). We assessed starvation survival, because changes in AKH signaling have previously been shown to impact starvation sensitivity. Genetic knockdown of three genes (Ca-Beta, Sur, and sei), in AKH producing cells caused highly significant changes (P < 0.001) in both male and female lifespan, and knockdown of six other genes (Shaw, cac, Ih, NaCP60E, stj, and TASK6) caused ...Continue Reading

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Citations

Apr 30, 2021·Insect Biochemistry and Molecular Biology·Umut Toprak, Laura Palanker Musselman
Jun 3, 2021·International Journal of Molecular Sciences·David Ben-Menahem
Jul 25, 2021·International Journal of Molecular Sciences·Jonathan M NelsonErik C Johnson
Jul 31, 2021·Current Biology : CB·Dennis PaulsChristian Wegener

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Datasets Mentioned

BETA
PRJNA642982

Methods Mentioned

BETA
sc-RNAseq
Transgenic
PCR

Software Mentioned

sc
Trimmomatic
TriKinetics Monitor
GenomicAlignments
Bioconductor package
RNAseq
DESeq2
biocondutor
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