Regulation of MHC gene expression during the differentiation of bone marrow-derived macrophages

Cellular Immunology
J K PullenL B Schook

Abstract

The ability of the macrophage to express class II MHC gene products appears to arise from both T-dependent and T-independent mechanisms. One mechanism by which macrophages express Ia-antigens in the absence of T-lymphocytes is postulated to be controlled by differentiation. By using a liquid bone marrow culture system, we have studied both class I and class II surface expression and mRNA accumulation during macrophage differentiation in vitro. The results demonstrated that Ia expression increased until 7 days in culture and then slowly declined. In contrast, class I expression appeared to steadily increase throughout the differentiation period. Northern blot analysis of RNA isolated from bone marrow-derived macrophages (BMDM) at various periods during culture, using E alpha, A alpha, and class I cDNA probes, correlated well with the results of Ia and H-2K surface expression. Further analysis demonstrated that the expression of Ia-antigens on BMDM was not the result of T-helper lymphocytes. This was determined by demonstrating (1) that bone marrow cultures were devoid of mature T-lymphocytes, (2) the absence of interferon (IFN)-gamma transcripts in both adherent and nonadherent populations of bone marrow cells, and (3) that the ...Continue Reading

References

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Citations

Sep 1, 1994·Pharmacology & Therapeutics·Y Ohmori, T A Hamilton
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A L Witsell, L B Schook
Jan 24, 2003·Infection and Immunity·Terri S HamrickPaul E Orndorff
Mar 1, 1995·Trends in Cell Biology·D G Russell

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