PMID: 7372712May 1, 1980Paper

Regulation of microtubule assembly in cultured fibroblasts

The Journal of Cell Biology
R E OstlundS V Hajek

Abstract

Microtubule assembly in diploid human skin fibroblasts was studied by [3H]colchicine binding to disaggregated microtubule subunits and to total cell tubulin. Microtubule content per milligram of cell protein was critically dependent upon cell density. As cultures neared confluence, microtubules increased and total cell tubulin decreased; the percent of tubulin assembled into microtubules increased from 5.3 in spare cultures to 58.3 in confluent cultures. Microtubules disappeared with a half-time of 2 min in response to 0 degree C incubation and reformed upon rewarming. Brief treatment of intact cells with concanavalin A or cytochalasin A depolymerized microtubules to 55 or 56% of control levels. The effect of concanavalin A was prevented by alpha-methylmannoside. Fibroblast microtubule assembly was not significantly altered by cyclic nucleotides, ascorbate, glucose, insulin, medium calcium concentration, or calcium ionophore A23187.

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Citations

Jan 1, 1995·Journal of Cellular Physiology·R HazanK L Crossin
Jan 24, 1991·Journal of Immunological Methods·D ThrowerL Wilson
Jan 1, 1983·Experimental Gerontology·M RaesJ Remacle
Jun 1, 1981·The Journal of Cell Biology·J B Olmsted
Apr 1, 1982·The Journal of Cell Biology·I Blikstad, L Carlsson
Mar 12, 2009·Journal of Biomechanical Engineering·Jason D HemmerMartine Laberge
Jul 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S M HallS G Haworth
Feb 23, 1981·FEBS Letters·L Carlsson, I Blikstad
Mar 1, 2006·Biotechnology and Bioengineering·Vy LamJohn Yin
Sep 1, 1981·Journal of Cellular Physiology·P J Del Vecchio, J R Smith
Sep 15, 2005·Experimental Cell Research·Barbara SigalaIrina R Tsaneva
Feb 12, 2015·PloS One·Victor S GoldmacherYelena V Kovtun
Jan 1, 1989·Immunopharmacology and Immunotoxicology·A Liepins

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