Regulation of microtubule organization during interphase and M phase

Cell Structure and Function
N Shiina, S Tsukita

Abstract

Microtubule (MT) dynamics and organization change markedly during interphase-M phase transition of the cell cycle. This mini review focuses first on p220, a ubiquitous MT-associated protein of Xenopus. p220 is phosphorylated by p34cdc2 kinase and MAP kinase in M phase, and concomitantly loses its MT-binding and MT-stabilizing activities. A cDNA encoding p220 was cloned, which identified p220 as a Xenopus homolog of MAP4, and p220 was therefore termed XMAP4. To examine the physiological relevance of XMAP4 phosphorylation during mitosis, Xenopus A6 cells were transfected with cDNA encoding wild-type or various XMAP4 mutants fused with a green fluorescent protein (GFP). Mutations of serine and threonine within potential phosphorylation sites for p34cdc2 kinase to nonphosphorylatable alanine interfered with mitosis-associated reduction in MT-affinity of XMAP4 and their overexpression affected chromosome movement during anaphase A. These results indicated that phosphorylation of XMAP4 by p34cdc2 kinase is responsible for the decrease in its MT-binding and MT-stabilizing activities during mitosis which are important for chromosome movement during anaphase A. The second focus is on a novel monoclonal antibody W8C3, which recognizes al...Continue Reading

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Citations

Jan 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·José A Sánchez-AlcázarPlácido Navas
Mar 31, 2009·Molecular Neurobiology·Orly Reiner, Tamar Sapir
Sep 19, 2015·Cytoskeleton·Manuel Oropesa ÁvilaJosé Antonio Sánchez-Alcázar
Jun 24, 2009·Cell Biochemistry and Function·Rong LiLin-Fang Wang

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