Regulation of mitotic spindle asymmetry by SUMO and the spindle-assembly checkpoint in yeast

Current Biology : CB
Christian LeisnerDimitris Liakopoulos

Abstract

During mitosis, the kinetochore microtubules capture and segregate chromosomes, and the astral microtubules position the spindle within the cell. Although the spindle is symmetric, proper positioning of the spindle in asymmetrically dividing cells generally correlates with the formation of morphologically and structurally distinct asters [1]. In budding yeast, the spindle-orientation proteins Kar9 and dynein decorate only one aster of the metaphase spindle and direct it toward the bud [2, 3]. The mechanisms controlling the distribution of Kar9 and dynein remain unclear. Here, we show that SUMO regulates astral-microtubule function in at least two ways. First, Kar9 was sumoylated in vivo. Sumoylation and Cdk1-dependent phosphorylation of Kar9 independently promoted Kar9 asymmetry on the spindle. Second, proper regulation of kinetochore function by SUMO was also required for Kar9 asymmetry. Indeed, activation of the spindle-assembly checkpoint (SAC) due to SUMO and kinetochore defects promoted symmetric redistribution of Kar9 in a Mad2-dependent manner. The control of Kar9 distribution by the SAC was independent of Kar9 sumoylation and phosphorylation. Together, our data reveal that three independent mechanisms contribute to Kar9...Continue Reading

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Citations

Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Christopher M HickeyMark Hochstrasser
Mar 31, 2012·The Journal of Biological Chemistry·Daniela HülsDierk Niessing
Jun 11, 2010·Molecular Biology of the Cell·Cristina Cepeda-GarcíaMarisa Segal
Nov 1, 2011·BMC Biology·Zachary C ElmoreOliver Kerscher
Apr 12, 2012·Genetics·Mark Winey, Kerry Bloom
Jul 6, 2010·Current Biology : CB·Daniel KammererDimitris Liakopoulos
Nov 13, 2012·Current Opinion in Cell Biology·Lea Stevermann, Dimitris Liakopoulos
Sep 30, 2014·FEMS Yeast Research·Ryo Higuchi-SanabriaLiza A Pon
Feb 26, 2016·Developmental Cell·Jörg SchweiggertDimitris Liakopoulos
Sep 30, 2016·Molecular Biology of the Cell·Cristina ManatschalMichel O Steinmetz
Nov 20, 2016·The Journal of Eukaryotic Microbiology·Bruno M Di GenovaRenata R Tonelli
May 8, 2018·Cytoskeleton·Matt GreenleeRita K Miller
May 18, 2018·Journal of Molecular Cell Biology·Zhixiong DongWei Jiang
Aug 3, 2019·Cells·Ariane Abrieu, Dimitris Liakopoulos

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