Regulation of motility of myogenic cells in filling limb muscle anlagen by Pitx2.

PloS One
Adam CampbellC Kioussi

Abstract

Cells of the ventrolateral dermomyotome delaminate and migrate into the limb buds where they give rise to all muscles of the limbs. The migratory cells proliferate and form myoblasts, which withdraw from the cell cycle to become terminally differentiated myocytes. The myogenic lineage colonizes pre-patterned regions to form muscle anlagen as muscle fibers are assembled. The regulatory mechanisms that control the later steps of this myogenic program are not well understood. The homeodomain transcription factor Pitx2 is expressed specifically in the muscle lineage from the migration of precursors to adult muscle. Ablation of Pitx2 results in distortion, rather than loss, of limb muscle anlagen, suggesting that its function becomes critical during the colonization of, and/or fiber assembly in, the anlagen. Microarrays were used to identify changes in gene expression in flow-sorted migratory muscle precursors, labeled by Lbx1(EGFP/+), which resulted from the loss of Pitx2. Very few genes showed changes in expression. Many small-fold, yet significant, changes were observed in genes encoding cytoskeletal and adhesion proteins which play a role in cell motility. Myogenic cells from genetically-tagged mice were cultured and subjected t...Continue Reading

References

Dec 1, 1992·Developmental Biology·E Bandman
Dec 1, 1988·The Journal of Cell Biology·L CassimerisE D Salmon
Dec 22, 1988·Nature·J B Gurdon
Sep 1, 1985·The Journal of Cell Biology·S A LewisN J Cowan
Oct 19, 1995·Nature·T Yagami-HiromasaA Fujisawa-Sehara
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L Y SzeD Paulin
Mar 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Y ZhangP Ekblom
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·N FreyE N Olson
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·F TakadaA H Beggs
Feb 24, 2001·Nature·J KawaiUNKNOWN RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
Apr 11, 2001·Trends in Biochemical Sciences·M ReinhardU Walter
Jul 13, 2001·Current Opinion in Genetics & Development·A Locascio, M A Nieto
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Feb 21, 2002·Molecular Biology of the Cell·Qize Wei, Robert S Adelstein
Mar 23, 2002·Science·Motoaki SekiKazuo Shinozaki

❮ Previous
Next ❯

Citations

Aug 25, 2012·Gene·Adam L CampbellChrissa Kioussi
Sep 4, 2012·Mutation Research·Nina KramerHelmut Dolznig
May 23, 2018·Journal of Developmental Biology·Chih-Ning Chang, Chrissa Kioussi
Nov 12, 2018·Developmental Biology·Chih-Ning ChangChrissa Kioussi
Apr 13, 2021·Cellular and Molecular Life Sciences : CMLS·Thai Q Tran, Chrissa Kioussi

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE31945

Methods Mentioned

BETA
fluorescence activated cell sorting
GTPases
GTPase
PCR
genotyping

Software Mentioned

MUT
GeneSpring
Cytoscape

Related Concepts

Related Feeds

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.