Regulation of mouse Scgb3a1 gene expression by NF-Y and association of CpG methylation with its tissue-specific expression

BMC Molecular Biology
Takeshi Tomita, Shioko Kimura

Abstract

Secretoglobin (SCGB) 3A1 is a secretory protein of small molecular weight with tumor suppressor function. It is highly expressed in lung and trachea in both human and mouse, with additional tissues expressing the protein that differ depending on the species. However, little is known about the function and transcriptional regulation of this gene in normal mouse tissues. By reporter gene transfection and gel mobility shift analyses, we demonstrated that expression of the mouse Scgb3a1 gene is regulated by a PU-box binding protein and a ubiquitous transcription factor NF-Y that respectively binds to the PU-boxes located at -99 to -105 bp and -158 to -164 bp, and the "CCAAT" binding sites located at -425 to -429 bp and -498 to -502 bp from the transcription start site of the gene. However, the effect of PU-box binding protein on transcriptional activation is minimal as compared to NF-Y, suggesting that NF-Y is a more critical transcription factor for mouse Scgb3a1 gene transcription. Despite that NF-Y is a ubiquitous factor, Scgb3a1 is highly expressed only in mouse lung and mtCC cells that are derived from SV40 transformed mouse Clara cells, but not in ten other mouse tissues/cells examined. Gene methylation analysis revealed that...Continue Reading

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Citations

Nov 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Takeshi TomitaShioko Kimura
May 23, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Di DaiXiao-Lin Guo
May 15, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Reiko KurotaniShioko Kimura
Nov 14, 2013·Journal of Cachexia, Sarcopenia and Muscle·Jvalini T DwarkasingKlaske van Norren
Nov 18, 2010·Molecular Biology Reports·Wangjun WuYuanzhu Xiong

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Methods Mentioned

BETA
transfection
immunoprecipitation
ChIP
PCR
Assay
protein assay
FCS
PCRs

Software Mentioned

BLAST
ImageQuant TL
TRANSFAC

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