Regulation of murein biosynthesis and septum formation in filamentous cells of Escherichia coli PAT 84.

Journal of Bacteriology
D MirelmanU Schwarz

Abstract

Both the beta-lactam antibiotic, cephalexin, and the deoxyribonucleic acid synthesis inhibitor, nalidixic acid, are known to inhibit cell division in Escherichia coli and induce the formation of filaments. The biosynthesis of murein was investigated in these filaments and compared with the murein synthesized by the normally dividing rods of E. coli PAT 84. Differences were found in the extent of peptide side-chain cross-linkage. Filamentous cells had higher extents of cross-linkages in their newly synthesized murein. Quantitative analyses of the D-alanine carboxypeptidase and transpeptidase reactions in the different cells revealed that the carboxypeptidase activity of the filamentous cells was partially inhibited. These results were similar to those previously found with filaments that were obtained after growth of the thermosensitive division mutant at its restrictive temperature. We conclude that the formation of new cell ends (septa) depends on the proper balance between the activities of the D-alanine carboxypeptidase that regulates the availability of precursor doners and the transpeptidase, which catalyzes cross-linking and attachment of newly synthesized murein.

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Citations

Mar 1, 1979·European Journal of Biochemistry·D Mirelman, Y Nuchamowitz
Jan 1, 1983·Journal of Receptor Research·J ZorzopulosL M Kozloff
Feb 23, 2008·FEMS Microbiology Reviews·Tanneke den BlaauwenJuan A Ayala
Apr 2, 2021·Nanoscale Advances·Preeyaporn SongkiatisakXiao-Hong Nancy Xu
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May 1, 1978·Journal of Bacteriology·D MirelmanE Z Ron
Jul 1, 1978·Antimicrobial Agents and Chemotherapy·J E Presslitz
Mar 1, 1982·Analytical Biochemistry·H Felix

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Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.