Regulation of murine lactate dehydrogenase C (Ldhc) gene expression

Biology of Reproduction
Huanghui TangErwin Goldberg

Abstract

Expression of Ldhc begins with the onset of meiosis in male germ cells and continues throughout spermatogenesis. Transcriptional regulatory mechanisms, especially in primary spermatocytes, are poorly described because of the lack of a reliable cell culture system. We constructed mouse transgenics and transfected germ cells in situ to study expression of the testis-specific isozyme of lactate dehydrogenase (LDH). From previous work, we determined that a 100-bp Ldhc core promoter contained potential cis regulatory elements, including a palindrome (-21 to +10), GC box (-70 to -65), and cAMP-responsive element (CRE) sites (-53 to -49, -39 to -35). We provide here the demonstration of a functional role for these sequences by expression of mutated transgenes in vivo. Our results reveal for the first time that mutation of the GC box does not abolish promoter activity, which remains testis-specific. Mutation of GC box or CRE sites resulted in a 73% and 74% reduction in promoter activity, respectively, in a transient transfection of germ cells in vivo by electroporation; the combination of GC box and CRE site mutations eliminates promoter activity. Therefore, we conclude that simultaneous occupancy of the GC box and CRE sites in the cor...Continue Reading

References

Jan 15, 2010·Nucleic Acids Research·Mirjana RajkovicJoachim M Weitzel
Sep 4, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Zhangsheng YangJohn R McCarrey
Mar 8, 2013·Biology of Reproduction·Huanghui TangErwin Goldberg

Citations

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May 4, 2002·Molecular and Cellular Endocrinology·Jeremy Don, Gil Stelzer
Aug 24, 2002·Biology of Reproduction·Kentaro YomogidaYoshitake Nishimune
Dec 21, 2002·Science·Betina MachoPaolo Sassone-Corsi
Oct 29, 2003·Journal of Andrology·Tim L KroftErwin Goldberg
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kiyoshi MikiDeborah A O'Brien
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji

Related Concepts

Pachytene
Neuro-Oncological Ventral Antigen 2
Cyclic AMP-Responsive DNA-Binding Protein
Benign Neoplasm of Testis
Galactosidase Activity
Transfection
Immunohistochemistry
Nuclear Proteins
CREM gene
Protein Methylation

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