PMID: 6109003Oct 1, 1980Paper

Regulation of muscarinic receptor-mediated cyclic GMP synthesis by cultured mouse neuroblastoma cells

Journal of Neurochemistry
E E El-Fakahany, E Richelson

Abstract

Mouse neuroblastoma clone N1E-115 has muscarinic acetylcholine receptors that mediate cyclic GMP synthesis. This receptor-mediated response is not significantly higher than background until the cells have been maintained in the stationary phase for at least 1 week. The basis of the influence of time in culture on the cyclic GMP response was investigated. The relative amount of cyclic GMP synthesized by intact cells was measured by radioactively labeling the GTP pool with [3H]guanine, incubating cells with agonists, and then chromatographically isolating [3H]cyclic GMP. Carbamylcholine-, ionophore X-537A-, and sodium azide-induced cyclic GMP formation increased with time in culture to a maximum of 13-, 9-, and 2.5-fold above basal, respectively. There was no change in the number or the apparent affinity of the muscarinic receptors as measured by [3H]quinuclidinyl benzylate ([3H]QNB) binding. In addition, there was no change in the apparent affinity of the receptors for agonist as measured by the ability of carbamylcholine to displace the specific binding of [3H]QNB. Guanylate cyclase activity per milligram protein and per cell increased six- and sevenfold, respectively, from day 0 to day 22. However, this increase in guanylate c...Continue Reading

References

Oct 15, 1978·The Biochemical Journal·T BartfaiP Greengard
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H Matsuzawa, M Nirenberg
Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H I Yamamura, S H Snyder

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Citations

Feb 1, 1994·Psychopharmacology·J HuE E el-Fakahany
Nov 1, 1981·Biochemical Pharmacology·E Richelson, E El-Fakahany
Oct 1, 1983·Biochemical Pharmacology·R K GordonP K Chiang
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R M SniderE Richelson
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T H LargeW L Klein
Jan 1, 1992·Annals of the New York Academy of Sciences·E Richelson
Jun 1, 1983·Journal of Neurochemistry·E El-FakahanyE Richelson
Mar 1, 1984·Journal of Neurochemistry·E E El-FakahanyE Richelson
Mar 1, 1983·Journal of Neurochemistry·E El-Fakahany, E Richelson
Feb 15, 2001·Biochemical Pharmacology·E JiménezM Montiel
Jan 28, 1982·Brain Research·H Repke, K Maderspach
Feb 25, 1991·European Journal of Pharmacology·B H SchmidtF A Sladeczek

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