Regulation of Na+ channels by luminal Na+ in rat cortical collecting tubule

The Journal of Physiology
L G PalmerG Frindt

Abstract

1. The idea that luminal Na+ can regulate epithelial Na+ channels was tested in the cortical collecting tubule of the rat using whole-cell and single-channel recordings. Here we report results consistent with the idea of Na+ self-inhibition. 2. Macroscopic amiloride-sensitive currents (INa) were measured by conventional whole-cell clamp. INa was a saturable function of external Na+ concentration ([Na+]o) with an apparent Km of 9 mM. Single channel currents (iNa) were measured in cell-attached patches. iNa increased with pipette Na+ concentration with an apparent Km of 48 mM. Since INa = (iNa)NPo, the different Km values imply that the channel density (N) and/or open probability (Po) increase as [Na+]o decreases. Reduction of [Na+]o after increasing intracellular Na+ concentration also increased the outward amiloride-sensitive conductance, consistent with activation of the Na+ channels. 3. The underlying mechanism was studied by changing pipette Na+ concentration while recording from cell-attached patches. No increase in NPo was observed, suggesting that the effect is not a direct interaction between [Na+]o and the channel. 4. [Na+]o was varied outside the patch-clamp pipette while recording from cell-attached patches. When amil...Continue Reading

References

Apr 1, 1988·Physiological Reviews·H Garty, D J Benos
Jan 1, 1983·The Journal of Membrane Biology·J H Li, B Lindemann
Jan 1, 1984·Annual Review of Physiology·B Lindemann
Feb 1, 1993·The American Journal of Physiology·A OharaD C Eaton
Jan 1, 1996·The Journal of General Physiology·L G Palmer, G Frindt
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·P KomwatanaD I Cook
Sep 1, 1995·The Journal of General Physiology·I I IsmailovD J Benos

❮ Previous
Next ❯

Citations

Feb 24, 2007·European Journal of Applied Physiology·Alexandra HebestreitHelge Hebestreit
Sep 18, 2007·Pflügers Archiv : European journal of physiology·Martin Fronius, Wolfgang G Clauss
Oct 26, 2010·Pflügers Archiv : European journal of physiology·Daniela Rotin, Olivier Staub
Jul 30, 2002·European Journal of Pharmacology·Timothy J BurtonDouglas R Ferguson
Aug 28, 1998·Current Opinion in Cell Biology·J D Horisberger
Jan 23, 2009·The Journal of Biological Chemistry·Ahmad B MaaroufShaohu Sheng
Oct 6, 1998·The Journal of General Physiology·G K Fyfe, C M Canessa
Aug 1, 2002·The Journal of General Physiology·Ahmed Chraïbi, Jean-Daniel Horisberger
Feb 22, 2005·American Journal of Respiratory and Critical Care Medicine·Sadis Matalon, Hong-Long Ji
Nov 26, 2008·BMC Biochemistry·Daniela Rotin
Oct 8, 2011·Journal of the American Society of Nephrology : JASN·Peter S Aronson, Gerhard Giebisch
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·H IshibashiD I Cook
Sep 3, 2002·The Journal of Physiology·Stephan KellenbergerLaurent Schild
Jul 6, 2010·Biochimica Et Biophysica Acta·Laurent Schild
Jul 24, 2003·The Journal of Biological Chemistry·Toru IshikawaDaniela Rotin
Jan 1, 2004·The Journal of Biological Chemistry·Shaohu ShengThomas R Kleyman
Dec 23, 2016·American Journal of Physiology. Renal Physiology·Tengis S Pavlov, Alexander Staruschenko
Nov 3, 2016·American Journal of Physiology. Renal Physiology·Evan C RayThomas R Kleyman
Feb 28, 2001·Biological Research for Nursing·L M Baxendale-Cox, R L Duncan
Aug 30, 2008·Journal of the American Society of Nephrology : JASN·Vivek Bhalla, Kenneth R Hallows
Dec 8, 2005·American Journal of Physiology. Renal Physiology·Jakob NielsenSøren Nielsen
Sep 13, 2002·American Journal of Physiology. Cell Physiology·N Yvonne Tallini, Larry C Stoner
Aug 30, 2013·American Journal of Physiology. Cell Physiology·Hector Rasgado-FloresRobert J Bridges
Jun 28, 2002·Physiological Reviews·Stephan Kellenberger, Laurent Schild
Apr 20, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·S Kunec, A Bose
Aug 14, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lukas Wichmann, Mike Althaus
May 15, 2003·American Journal of Physiology. Renal Physiology·Jonathan LebowitzJohn P Johnson
Oct 25, 2003·American Journal of Physiology. Cell Physiology·A InagakiT Ishikawa
Jan 3, 2001·American Journal of Physiology. Renal Physiology·G FrindtL G Palmer
Sep 13, 2001·American Journal of Physiology. Renal Physiology·L DijkinkR J Bindels
Aug 3, 2007·American Journal of Physiology. Renal Physiology·Vincent Bize, Jean-Daniel Horisberger
May 24, 2003·The Journal of Biological Chemistry·Elena BabiniStefan Gründer
Jul 12, 2002·American Journal of Physiology. Renal Physiology·James A Schafer
Aug 13, 1999·The American Journal of Physiology·M S Awayda
Aug 5, 2000·American Journal of Physiology. Renal Physiology·J LoffingB Kaissling
Aug 23, 2002·Journal of the American Society of Nephrology : JASN·Olivier Bonny, Bernard C Rossier
Nov 8, 2002·Biochimica Et Biophysica Acta·Mikael SchnizlerWolfgang Clauss
Jun 19, 2021·The Journal of General Physiology·Lei YangLawrence G Palmer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.