Regulation of Na channels in the rat cortical collecting tubule: effects of cAMP and methyl donors

The American Journal of Physiology
Gustavo Frindt, Lawrence G Palmer

Abstract

The whole cell patch-clamp technique was used to investigate the interactions of the amiloride-sensitive Na channel of the rat cortical collecting tubule (CCT) with adenosine 3',5'-cyclic monophosphate (cAMP) and with methyl donors. The amiloride-sensitive whole cell current (INa) was measured in principal cells of dissected, split-open tubules from rats maintained either on a control diet or on a low-Na diet to increase endogenous aldosterone secretion. With Na-depleted animals, INa was highest immediately after rupture of the membrane patch and averaged 325 pA at a membrane potential of -60 mV. INa declined over 15 min to approximately 35% of the initial value. With 8-(4-chlorophenylthio)-cAMP in the pipette, INa increased within 5 min of membrane rupture and was maintained for 15 min at levels three- to fourfold higher than the corresponding control values. With Na-replete animals, INa was undetectable (< 10 pA) without cAMP. With cAMP in the pipette, INa averaged 40 pA. In cell-attached patches on tubules from Na-replete rats exposed to cAMP, single Na channels were observed with conductive and kinetic properties similar to those from Na-depleted rats but at lower density. Inclusion of the methyl donor S-adenosyl methionine...Continue Reading

Citations

Dec 6, 2003·American Journal of Physiology. Renal Physiology·Gustavo Frindt, Lawrence G Palmer
Jul 12, 2002·American Journal of Physiology. Renal Physiology·James A Schafer
Oct 25, 2003·American Journal of Physiology. Cell Physiology·A InagakiT Ishikawa
Aug 15, 2001·American Journal of Physiology. Cell Physiology·J D StockandD C Eaton
Apr 6, 2000·American Journal of Physiology. Renal Physiology·J LoffingB Kaissling
Aug 5, 2000·American Journal of Physiology. Renal Physiology·J LoffingB Kaissling
Oct 20, 2005·American Journal of Physiology. Renal Physiology·Yuan WeiWen-Hui Wang
Jun 28, 2002·Physiological Reviews·Stephan Kellenberger, Laurent Schild
Apr 10, 2003·American Journal of Physiology. Renal Physiology·Anke DahlmannLawrence G Palmer

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