Regulation of Na+, K(+)-ATPase in the rat outer medullary collecting duct during potassium depletion.

Journal of the American Society of Nephrology : JASN
B Buffin-MeyerA Doucet

Abstract

Because in outer medullary collecting ducts (OMCD) of K(+)-depleted rats, K+ secretion is abolished, whereas Na+, K(+)-ATPase, which energizes this secretion, is markedly stimulated, it has been proposed that Na+, K(+)-ATPase was mislocated to the apical cell membrane and energized K+ reabsorption. This hypothesis has been supported by paradoxical effects of ouabain in K(+)-depleted compared with normal rats. However, we have recently shown that ouabain inhibits not only Na+, K(+)-ATPase but also apical H+, K(+)-ATPase in the OMCD of K(+)-depleted rats. Therefore, this study was designed to evaluate whether previous observations were accounted for by Na+, K(+)-ATPase or by ouabain-sensitive H+, K(+)-ATPase. Na+, K(+)-ATPase was distinguished from H+, K(+)-ATPase by its insensitivity to Sch-28080. Results indicate that the hydrolytic and transport activities of Na+, K(+)-ATPase, the number of its functional units, and the expression of mRNA of its alpha 1 and beta 1 subunits were increased threefold or more in the OMCD of rats fed a K(+)-depleted diet for 2 wk. By immunofluorescence, Na+, K(+)-ATPase staining was strongly increased in K(+)-depleted rats but remained localized to the basolateral pole of OMCD principal cells. In c...Continue Reading

Citations

Oct 25, 2001·Journal of the American Society of Nephrology : JASN·Georges DeschênesAlain Doucet
May 8, 1999·Journal of the American Society of Nephrology : JASN·I SabolićD Brown
Jul 9, 2004·Physiological Genomics·Lydie ChevalAlain Doucet
Feb 19, 2005·Neurobiology of Aging·C MasseguinJ Gabrion

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