Regulation of Na+,K+-ATPase alpha-subunit expression by mechanical strain in aortic smooth muscle cells

Hypertension
E Songu-MizeL J Hymel

Abstract

We have previously demonstrated that vascular sodium pump activity is stimulated in several rat models of hypertension. In addition, others have reported an upregulation of mRNA for the Na+,K+-ATPase alpha1-subunit in hypertension. To test the effect of sustained, cyclic, stretch-relaxation stimuli on the expression of alpha1- and alpha2-subunits of Na+,K+-ATPase in vascular smooth muscle cells, we used the Flexercell strain unit to stretch rat aortic smooth muscle cells for several days on a collagen-coated silicone elastomer substratum. Six-second cycles of stretch-relaxation were applied to obtain 10% average surface elongation (22% maximum) for 4 days. Control cells were not stretched but were grown on a similar surface. The effect of Gd3+, a blocker of stretch-activated channels, was also investigated. At the end of 4 days, protein expression of alpha1- and alpha2-subunits was determined by Western blot analysis. Intensity of the bands for alpha1- and alpha2-subunits was quantified with the use of a computerized image analyzer. In the stretched cells, both the alpha1- and the alpha2-subunit protein-band intensities were significantly increased compared with those of the non-stretched cells. Treatment with 50 micromol/L Gd3...Continue Reading

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Citations

Jan 3, 2001·Journal of Cellular Biochemistry·O HanB E Sumpio
Sep 28, 1998·The American Journal of the Medical Sciences·E Songu-MizeL J Hymel
May 16, 2000·Journal of Cardiovascular Electrophysiology·O BarbeyJ M Maixent
Jul 29, 2010·Clinical and Experimental Hypertension : CHE·Sarah Hoffmann Lindsey, Emel Songu-Mize
Feb 20, 2004·British Journal of Pharmacology·Patricia E M MartinTudor M Rachael J
Dec 4, 2003·British Journal of Pharmacology·Patricia E M MartinTudor M Griffith
Jul 27, 2006·Journal of Biomechanics·Jason H HagaShu Chien
Aug 13, 1999·Journal of Applied Physiology·C M WatersJ I Sznajder
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Madhumita Jena Mohanty, Xingli Li
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·E Songu-MizeM Jacobs
May 15, 2001·American Journal of Physiology. Cell Physiology·N SevieuxE Songu-Mize

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