PMID: 8948457Jun 15, 1995Paper

Regulation of neurite outgrowth from differentiated human neuroepithelial cells: a comparison of the activities of prothrombin and thrombin

The Biochemical Journal
A S TurnellR J Grand

Abstract

The mechanism by which thrombin and prothrombin control neurite retraction was studied in Ad12E1HER10 human neuroepithelial cells. Morphological changes in differentiated cells were apparent within minutes of the addition of very low concentrations of thrombin (3 pM). Higher concentrations (2 nM) of prothrombin were required to elicit a similar response. Doses of thrombin and prothrombin sufficient to cause neurite retraction stimulated protein tyrosine kinase activity. Protein tyrosine kinase activation also correlated positively with thrombin- and prothrombin-induced phosphoinositide 3-kinase activation and InsP6 dephosphorylation. However, thrombin-stimulated Ins(1,4,5)P3 generation and intracellular Ca2+ mobilization only occurred at concentrations in excess of those needed to induce retraction. No fluctuations in Ins(1,4,5)P3 were detected after stimulation with prothrombin, and no rapid synchronized release of Ca2+ was observed, even at very high concentrations. Prothrombin did, however, cause small oscillations in the intracellular Ca2+ concentration, similar to those produced by low concentrations of thrombin, after approximately 30 min. We conclude that prothrombin- and thrombin-induced neurite retractions are not depe...Continue Reading

Citations

Jul 5, 2005·Experimental Neurology·Mark A ParkerEvan Y Snyder
Apr 23, 2009·Neuroscience Letters·Lakshmi ThirumangalakudiPaula Grammas
May 20, 1998·Journal of Molecular Neuroscience : MN·I V SmirnovaB W Festoff
Apr 5, 2003·Biological Chemistry·Hong Wang, Georg Reiser
Dec 22, 2019·International Journal of Biological Macromolecules·Sonali R BhagwatAmit Kumar

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