Regulation of neuroblast proliferation by surface glia in the Drosophila larval brain

Scientific Reports
Makoto I KanaiHiroshi Nakato

Abstract

Despite the importance of precisely regulating stem cell division, the molecular basis for this control is still elusive. Here, we show that surface glia in the developing Drosophila brain play essential roles in regulating the proliferation of neural stem cells, neuroblasts (NBs). We found that two classes of extracellular factors, Dally-like (Dlp), a heparan sulfate proteoglycan, and Glass bottom boat (Gbb), a BMP homologue, are required for proper NB proliferation. Interestingly, Dlp expressed in perineural glia (PG), the most outer layer of the surface glia, is responsible for NB proliferation. Consistent with this finding, functional ablation of PG using a dominant-negative form of dynamin showed that PG has an instructive role in regulating NB proliferation. Gbb acts not only as an autocrine proliferation factor in NBs but also as a paracrine survival signal in the PG. We propose that bidirectional communication between NBs and glia through TGF-β signaling influences mutual development of these two cell types. We also discuss the possibility that PG and NBs communicate via direct membrane contact or transcytotic transport of membrane components. Thus, our study shows that the surface glia acts not only as a simple structu...Continue Reading

References

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Citations

Dec 14, 2019·Genes & Development·Maricela Robles-MurguiaFabio Demontis
Aug 20, 2020·Journal of Neuroscience Research·Asif Bakshi, Rohit Joshi
Oct 24, 2018·Journal of Developmental Biology·Katherine Harding, Kristin White
Nov 18, 2018·Glia·Kerem YildirimChristian Klämbt
Nov 28, 2019·Glycoconjugate Journal·Shoko Nishihara
Aug 28, 2020·Frontiers in Oncology·Zoya Yan, Shanzhi Wang
Jul 23, 2020·Frontiers in Cell and Developmental Biology·Helena Mira, Javier Morante
Jan 1, 2021·Frontiers in Cell and Developmental Biology·Inês Lago-BaldaiaSarah D Ackerman
Jun 4, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mengying YangMargaret S Ho

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Methods Mentioned

BETA
co-immunoprecipitation
transgenic
transfections
PCR

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