PMID: 7544448Apr 1, 1995Paper

Regulation of neuronal nitric oxide synthase by histone, protamine, and myelin basic protein

Neurochemical Research
J HuE E el-Fakahany

Abstract

We examined the effects of endogenous basic proteins rich in the amino acid L-arginine on neuronal NO synthase activity by monitoring cyclic GMP formation in intact neuron-like neuroblastoma N1E-115 cells. Histone, protamine and myelin basic protein significantly stimulated cyclic GMP formation, both in a time- and concentration-dependent manner. These effects were blocked by hemoglobin and NO synthase inhibitors. Removal of the extracellular/intracellular Ca2+ gradient by a Ca2+ chelator completely abolished the cyclic GMP responses elicited by histone and protamine, suggesting that influx of extracellular Ca2+ might be involved in their activation of NO synthase. The effects of myelin basic protein on cyclic GMP formation, however, appeared to be due to Ca2+ release from intracellular stores. In cytosolic preparations of rat cerebellum, these basic proteins inhibited the metabolism of L-arginine into L-citrulline by NO synthase. We conclude from our findings that endogenous basic proteins might be involved in the regulation of neuronal NO synthase activity. Their effects on the enzyme could be either stimulatory or inhibitory, depending on whether the basic proteins exert their effects extracellularly or intracellularly, resp...Continue Reading

References

Dec 9, 1991·FEBS Letters·M HeckerJ R Vane
Mar 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L D GorskyF Murad
Jan 16, 1989·Biochemical and Biophysical Research Communications·G ThomasP W Ramwell
Oct 1, 1989·Japanese Journal of Pharmacology·T Suzuki-NishimuraM K Uchida
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E El-Fakahany, E Richelson
Jul 4, 1994·Neuroscience Letters·J HuE E el-Fakahany
Feb 1, 1994·Psychopharmacology·J HuE E el-Fakahany
Aug 18, 1993·Biochimica Et Biophysica Acta·H H SchmidtU Walter
Jul 1, 1993·The Journal of Experimental Medicine·N SuttorpS Bhadki
Mar 1, 1993·Journal of Vascular Research·W R Tracey, M J Peach

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Citations

Nov 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Hymes, B Wolf
Aug 5, 2003·Peptides·Oscar A BrownRodolfo G Goya
Oct 30, 1999·Alimentary Pharmacology & Therapeutics·Y Li, C H Cho
Apr 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H Kinoshita, Z S Katusic
Mar 4, 1999·The Journal of Nutrition·J Hymes, B Wolf

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