Regulation of NF-κB by the p105-ABIN2-TPL2 complex and RelAp43 during rabies virus infection

PLoS Pathogens
Benoit BessonHervé Bourhy

Abstract

At the crossroad between the NF-κB and the MAPK pathways, the ternary complex composed of p105, ABIN2 and TPL2 is essential for the host cell response to pathogens. The matrix protein (M) of field isolates of rabies virus was previously shown to disturb the signaling induced by RelAp43, a NF-κB protein close to RelA/p65. Here, we investigated how the M protein disturbs the NF-κB pathway in a RelAp43-dependant manner and the potential involvement of the ternary complex in this mechanism. Using a tandem affinity purification coupled with mass spectrometry approach, we show that RelAp43 interacts with the p105-ABIN2-TPL2 complex and we observe a strong perturbation of this complex in presence of M protein. M protein interaction with RelAp43 is associated with a wide disturbance of NF-κB signaling, involving a modulation of IκBα-, IκBβ-, and IκBε-RelAp43 interaction and a favored interaction of RelAp43 with the non-canonical pathway (RelB and p100/p52). Monitoring the interactions between host and viral proteins using protein-fragment complementation assay and bioluminescent resonance energy transfer, we further show that RelAp43 is associated to the p105-ABIN2-TPL2 complex as RelAp43-p105 interaction stabilizes the formation of a ...Continue Reading

References

Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·S C SunW C Greene
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Mar 27, 2003·The Journal of Biological Chemistry·Ralf MarienfeldManfred Neumann
May 8, 2003·The Journal of Biological Chemistry·Cyril Couturier, Ralf Jockers
Jan 27, 2004·Nature Cell Biology·Tewis BouwmeesterGiulio Superti-Furga
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Barbara MarinariLoretta Tuosto
Oct 19, 2004·Shock·Hiroyuki WatanabeAkihiro Matsukawa
Sep 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Emilie JacqueVéronique Baud
Apr 25, 2006·Nature Immunology·Stamatia PapoutsopoulouSteven C Ley
Jan 16, 2007·BMC Immunology·Omar SharifNeil D Perkins
Mar 7, 2008·Journal of Virology·Alireza GholamiHervé Bourhy
Dec 30, 2008·PLoS Pathogens·Stephen C GrahamHervé Bourhy
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Andrea Oeckinghaus, Sankar Ghosh
Feb 5, 2010·Journal of Virology·Tatsunori MasataniMakoto Sugiyama
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Ellen O'Dea, Alexander Hoffmann
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
May 24, 2011·Advances in Virus Research·Atsushi Okumura, Ronald N Harty
Dec 1, 2011·Nature Methods·Patricia CassonnetYves Jacob
Jun 29, 2012·Journal of Virology·L WiltzerG W Moseley
Oct 11, 2012·Cellular Microbiology·Reiko PollinStefan Finke
Aug 21, 2013·Nature Reviews. Immunology·J Simon C Arthur, Steven C Ley
Aug 31, 2013·Nature Reviews. Molecular Cell Biology·Kristopher ClarkPhilip Cohen
Dec 25, 2013·The Journal of Infectious Diseases·Linda WiltzerGregory William Moseley
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Apr 5, 2016·The Journal of Veterinary Medical Science·Naoto ItoMakoto Sugiyama

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Citations

Aug 23, 2019·Scientific Reports·Florian SonthonnaxHervé Bourhy
Sep 10, 2018·Archivum Immunologiae Et Therapiae Experimentalis·Justyna Struzik, Lidia Szulc-Dąbrowska
Nov 25, 2020·Acta Neuropathologica Communications·Madlin PotratzStefan Finke
Nov 14, 2019·Cell Reports·Md Alamgir HossainPaul R Gooley
Jan 31, 2021·Molecular & Cellular Proteomics : MCP·Alice MeigniéAnastassia V Komarova
May 18, 2021·Frontiers in Medicine·Shaun PienkosVinicio A de Jesús Pérez

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Methods Mentioned

BETA
ubiquitination
tandem affinity purification
BRET
PCA
transfection
co-immunoprecipitation
PCR
acetylation
X-ray

Software Mentioned

7500 SDS
Maxquant
GraphPad Prism
Perseus
STRING
XCalibur
Andromeda
Cytoscape
DAVID

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