PMID: 8611615Apr 10, 1996Paper

Regulation of NHE1 expression in L6 muscle cells

Biochimica Et Biophysica Acta
W YangLarry Fliegel

Abstract

We examined regulation of expression of the NHE1 promoter on rat L6 cells. Transient transfection of these cells showed that there are two regions critical for basal expression in this cell type. One is from bp -155 to -171 and second more proximal region is between bp -92 and -125. When cells were induced to differentiate by serum withdrawal, mRNA levels rose 2-3-fold. To investigate the mechanisms of this phenomenon a series of stable transfectants were made of the NHE1 promoter in L6 cells. Muscle differentiation caused a significant stimulation of transcriptional activity in the stable cells containing the more distal regions of the promoter. The results show that basal expression of the NHE1 promoter is mediated largely by two proximal regions of the gene. However, during the process of differentiation more distal regions of the gene are involved in elevation of the level of expression.

References

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Citations

Jan 15, 2008·Journal of Molecular and Cellular Cardiology·Larry Fliegel
Jun 16, 2007·Cell Death and Differentiation·A P KumarM-V Clément
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Emily Slepkov, Larry Fliegel
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Carmen V Rieder, Larry Fliegel
Jan 11, 2007·The Biochemical Journal·Emily R SlepkovLarry Fliegel
Nov 18, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Xiuju LiLarry Fliegel

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