Regulation of nitrogen utilization of hisT mutants of Salmonella typhimurium.

Journal of Bacteriology
S A Rosenfeld, J E Brenchley

Abstract

Mutations in the hisT gene of Salmonella typhimurium alter pseudouridine synthetase I, the enzyme that modifies two uridines in the anticodon loop of numerous transfer ribonucleic acid species. We have examined two strains carrying different hisT mutations for their ability to grow on a variety of nitrogen sources. The hisT mutants grew more rapidly than did hisT+ strains with either arginine or proline as the nitrogen source and glucose as the carbon source. The hisT mutations were transduced into new strains to show that these growth properties were due to the hisT mutations. The hisT mutations did not influence the growth of mutants having altered glutamine synthetase regulation. Assays of the three primary ammonia-assimilatory enzymes, glutamate dehydrogenase, glutamine synthetase, and glutamate synthase, showed that glutamate synthase activities were lower in hisT mutants than in isogenic hisT+ controls; however, the glutamate dehydrogenase activity was about threefold higher in the hisT strains grown in glucose-arginine medium. The results suggest that the controls for enzyme synthesis for nitrogen utilization respond either directly or indirectly to transfer ribonucleic acid species affected by the hisT mutation.

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Citations

Jan 1, 1982·Molecular & General Genetics : MGG·M Sales, J E Brenchley
Jun 1, 1993·Molecular & General Genetics : MGG·A V OsorioF Bastarrachea
Jan 1, 1984·Molecular & General Genetics : MGG·S A RosenfeldN W Ho
May 1, 2014·EcoSal Plus·Glenn R Björk, Tord G Hagervall
May 1, 1982·Journal of Bacteriology·S A RosenfeldJ E Brenchley
Sep 1, 1983·Microbiological Reviews·K E Sanderson, J R Roth
Nov 1, 2005·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Jan 1, 1985·Journal of Bacteriology·M J MadonnaJ E Brenchley
Jan 1, 1985·Journal of Bacteriology·C C MarvelM E Winkler

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