Abstract
By using monoclonal antibody specific for tumor necrosis factor receptor80 (TNFR80) (CD120b) and TNFR60 (CD120a), we determined which receptor transduces the signals involved in activating natural killer (NK) cells. Purified CD56+CD3- large lymphocytes express TNFR80 but not TNFR60 and interleukin-2 (IL-2) up-regulates TNFR80 expression, consistent with NK cells being activated in vivo. Treatment of NK cells with anti-TNFR80 for 18 h enhanced the NK activity detected on K562 target cells mimicking the effect of TNF. In combination with IL-2, TNF enhanced the development of lymphokine-activated killing. However, only anti-TNFR80 abrogated IL-2 induction of lymphokine-activated killer cell activity. The activity of TNF or anti-TNFR80 was selective for NK cytotoxic function because they did not directly mimic IL-2 activation or induce significant proliferation, expression of cell surface activation antigens (CD25 or HLA-DR), or interferon-gamma secretion. These results indicate that TNFR80 is an important signal transducing receptor for the differentiation of NK cells induced by TNF and IL-2.
Citations
Mar 13, 1999·Critical Care Medicine·W HüblP M Bayer
Nov 11, 1999·European Journal of Immunology·G CaronP Jeannin
Mar 30, 2001·The Journal of Biological Chemistry·C SeitzT Hehlgans
Sep 29, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T HehlgansD N Männel
Jun 22, 2005·The Journal of Experimental Medicine·Mark J SmythYoshihiro Hayakawa
Jun 16, 2004·Journal of Leukocyte Biology·Balazs TothMartin G Schwacha
Jul 13, 2000·Microscopy Research and Technique·M J Smyth, R W Johnstone
Feb 13, 2001·Tissue Antigens·M TakahashiA Kimura
Dec 14, 2006·Blood·Jun XuNikola L Vujanovic
Aug 31, 2018·Arthritis Research & Therapy·Ursula Schulte-WredeAndreas Grützkau
May 1, 2010·Blood·Lazar VujanovicLisa H Butterfield
Apr 29, 2021·ImmunoTargets and Therapy·Yang YangXin Chen
Aug 13, 1999·Molecular Genetics and Metabolism·K M Cunnion
Mar 21, 1998·Genomics·C SeitzT Hehlgans
Jul 6, 2005·Journal of Inflammation·Christoph ScherüblDaniela N Männel