PMID: 9537832Apr 16, 1998Paper

Regulation of oligodendrocyte differentiation: protein kinase C activation prevents differentiation of O2A progenitor cells toward oligodendrocytes

Glia
Wia BaronDick Hoekstra

Abstract

Oligodendrocytes differentiate on a specific schedule in vivo in order to myelinate axons at the precise time and at the appropriate position. The current study was undertaken to obtain further insight as to how this timed appearance is regulated intracellularly. We observed that exposure of O2A progenitor cells in culture to phorbol 12-myristate 13-acetate (PMA; an activator of protein kinase C, PKC) inhibited their differentiation to oligodendrocytes by suppressing the expression of specific myelin markers at the O4-stage. To positively identify a role of PKC per se in differentiation, the use of a minimal medium with low serum content turned out to be essential. This was demonstrated by showing that the inhibitory effect of PMA on oligodendrocyte differentiation could be completely abolished by a combined action of insulin, triiodothyronine (T3), hydrocortisone and other components of a chemically defined medium (CDM). Furthermore, the PMA-mediated inhibition of oligodendrocyte differentiation could be partially restored by activation of the cAMP signal transduction pathway. The results indicate that PKC plays a crucial role in the differentiation of O2A progenitor cells toward oligodendrocytes: PKC activation prevents diffe...Continue Reading

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Citations

Mar 29, 2001·Microscopy Research and Technique·R L Stariha, S U Kim
Jan 4, 2008·Journal of Molecular Neuroscience : MN·Olaf MaierWia Baron
May 3, 2002·Neuropharmacology·Amani KhorchidGuillermina Almazan
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May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pablo M PaezAnthony T Campagnoni
Nov 3, 2007·Journal of Neuroscience Research·Bert KlunderDick Hoekstra
Feb 9, 2005·Experimental Neurology·Arthur M Butt, Jennifer Dinsdale
Jan 31, 2006·Toxicology and Applied Pharmacology·Nukhet Aykin-Burns, Nuran Ercal
Jul 18, 2009·Molecular and Cellular Neurosciences·Zuzana SiskováWia Baron
Oct 20, 2006·Neuroscience·R Káradóttir, D Attwell
Jul 12, 2002·Glia·Francesca BlasiMarialuisa Melli
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Oct 6, 2017·Frontiers in Pharmacology·Kazushige Mizoguchi, Yasushi Ikarashi
Apr 4, 2021·International Journal of Molecular Sciences·Simona DamianoMariarosaria Santillo

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