PMID: 6989407Apr 17, 1980Paper

Regulation of ornithine decarboxylase in cultured mouse mammary gland by the osmolarity in the cellular environment

Biochimica Et Biophysica Acta
J W Perry, T Oka

Abstract

The biphasic increase of ornithine decarboxylase activity in mouse mammary gland in organ culture occurs with a hormone-independent first peak and a hormone-dependent second peak. The data presented indicate that a change in the osmolarity of the cellular environment is the major contributing factor for the emergence of the hormone-independent ornithine decarboxylase activity in mammary explants. Thus, incubation of mammary explants for 3 h in a medium diluted 53% with distilled water results in approx. 1000-fold stimulation of enzyme activity over the initial level, whereas a similar dilution of the medium with 0.18 M NaCl or 0.3 M sucrose blocks the increase. The increase in enzyme activity is similarly affected by a reduction of the concentration of NaCl in the culture medium. The hypoosmotic stimulation of ornithine decarboxylase activity appears to be affected at a posttranscriptional level, and is enhanced further by the actions of insulin and prolactin. The hypoosmotic enhancement of ornithine decarboxylase activity produces a large increase in the intracellular concentration of putrescine in mammary explants. However, neither the concentration of spermidine and spermine nor the activity of S-adenosyl-L-methionine decarb...Continue Reading

Citations

Feb 1, 1988·Journal of Steroid Biochemistry·W S BranhamD M Sheehan
May 1, 1995·The International Journal of Biochemistry & Cell Biology·A E PeggJ K Coward
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
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Jul 18, 1984·Biochemical and Biophysical Research Communications·R PoulinP Nadeau
Jan 15, 1982·Biochemical and Biophysical Research Communications·T J Paulus, R H Davis

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