PMID: 8952025Nov 1, 1996Paper

Regulation of P-selectin expression by inflammatory mediators in canine jugular endothelial cells

Veterinary Pathology
M Doré, J Sirois

Abstract

Canine endothelial cells express the adhesion molecule P-selectin to mediate the initial attachment of leukocytes to the vessel wall. Although it is known that agents like histamine and thrombin stimulate the surface expression of P-selectin, the effect of inflammatory mediators and cytokines such as lipopolysaccharides (LPS), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) on canine P-selectin expression has not been investigated. Therefore, the objective of this study was to analyze the regulation of P-selectin messenger RNA (mRNA) and protein by these cytokines in canine endothelial cells isolated from jugular veins. Analyses of cytoplasmic RNA by Northern blotting showed that stimulation of culture endothelial cells with either LPS (100 ng/ml) or recombinant human TNF-alpha (30 U/ml) for 3 or 6 hours significantly increased (P < 0.05) steady-state levels of mRNA for P-selectin (3.8- +/- 1.0- and 3.0- +/- 0.4-fold increase for LPS at 3 and 6 hours, respectively, and 2.5- +/- 0.8- and 2.7- +/- 0.9-fold increase for TNF-alpha at 3 and 6 hours, respectively). P-selectin mRNA had decreased by 48 hours to levels found in unstimulated cells. In contrast, human IL-1 beta had no effect on P-selectin mRNA....Continue Reading

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Citations

Dec 15, 2010·The Journal of Experimental Medicine·Zhenghui LiuRodger P McEver
Nov 2, 2007·Clinical and Experimental Pharmacology & Physiology·Shu-Qin YingJian-An Wang
Jul 13, 2006·Journal of Ethnopharmacology·Pisamai LaupattarakasemChariya Hahnvajanawong
Jan 3, 2012·European Journal of Pharmacology·Kuncha MadhusudanaPrakash V Diwan
Jun 1, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Chattopadhyay DebprasadChakraborti Sekhar
Aug 24, 2006·Journal of Vascular Research·Rainer KiefmannAlwin Eduard Goetz
Apr 23, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Nan ZhangRodger P McEver
Feb 18, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Douglas H ThammDavid M Vail

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