Regulation of p53 expression and apoptosis by vault RNA2-1-5p in cervical cancer cells

Oncotarget
Lu KongYu-Xiang Zhang

Abstract

nc886 or VRNA2-1 has recently been identified as a noncoding RNA instead of a vault RNA or a pre-microRNA. Several studies have reported that pre-miR-886 plays a tumor-suppressive role in a wide range of cancer cells through its activity as a cellular protein kinase RNA-activated (PKR) ligand and repressor. However, by sequencing stem-PCR products, we found that a microRNA originating from this precursor, vault RNA2-1-5p (VTRNA2-1-5p), occurs in cervical cancer cells. The expression levels of the predicted targets of VTRNA2-1-5p are negatively correlated with VTRNA2-1-5p levels by quantitative reversion transcription PCR (qRT-PCR). Previous results have shown that VTRNA2-1-5p is overexpressed in human cervical squamous cell carcinomas (CSCCs) compared with adjacent healthy tissues. Inhibition of VTRNA2-1-5p increases Bax protein expression and apoptotic cell death in cervical cancer cells. Our findings suggest that VTRNA2-1-5p has oncogenic activity related to the progression of cervical cancer. Here, we report that VTRNA2-1-5p directly targeted p53 expression and functioned as an oncomir in cervical cancer. VTRNA2-1-5p inhibition decreased cervical cancer cell invasion, proliferation, and tumorigenicity while increasing apopto...Continue Reading

References

Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·J FairmanL Nagarajan
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T D KessisK R Cho
Aug 5, 1998·Pharmacology & Therapeutics·M Scheffner
Mar 31, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T G HuangH Y Ngan
Mar 3, 2004·Journal of Cellular Biochemistry·Haimei HuangTer-Mei Huang
Aug 9, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Inbar FriedrichAlexander Levitzki
May 9, 2008·Nature·Yoshinori KawamuraHaruhiko Siomi
Sep 2, 2008·Cellular and Molecular Life Sciences : CMLS·W BergerM Micksche
Mar 21, 2009·Journal of Molecular Biology·Constanze NandyNorbert Polacek
May 28, 2009·Cell Biochemistry and Function·Hong-Joo AnSang-Chul Park
Jun 6, 2009·Molecular Biology and Evolution·Peter F StadlerKerstin Ullmann
Dec 8, 2009·Nucleic Acids Research·Jian-Hua YangLiang-Hu Qu
Jul 14, 2010·Oncogene·V Olivares-Illana, R Fåhraeus
Dec 24, 2010·PloS One·Manoj M PillaiBeverly Torok-Storb
Mar 29, 2011·Archives of Virology·Wenyi GuAnnika Antonsson
Jan 17, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Gerda HofstetterNicole Concin
Jul 5, 2012·PloS One·Martina SchmitzMatthias Dürst
Jul 24, 2012·Carcinogenesis·Dawid WalerychGiannino Del Sal
Jul 31, 2012·Nucleic Acids Research·Anne DueckGunter Meister

❮ Previous
Next ❯

Citations

Aug 23, 2018·The Prostate·Pierre-Antoine DuguéMelissa C Southey
Sep 11, 2018·Cancer Biomarkers : Section a of Disease Markers·Wei-Yi ZhangPing Chen
Aug 24, 2019·The Journal of Biological Chemistry·Nikolay G KolevChristian Tschudi
Feb 25, 2020·The EMBO Journal·Ruth L SealElspeth A Bruford
Dec 5, 2020·Critical Reviews in Oncology/hematology·Xiangxuan ZhaoZaiming Lu

❮ Previous
Next ❯

Datasets Mentioned

BETA
JN900492.1

Methods Mentioned

BETA
immunoprecipitation
anti-AGO RIP
transfection
PCR
Xenografts
flow cytometry
RIP
Assay
xenograft

Software Mentioned

Wallac EnVision Manager
ImageJ
SPSS
VTRNA

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis