Regulation of peroxisome proliferator activated receptor alpha-mediated pathways in alcohol fed cytochrome P450 2E1 deficient mice

Hepatology Research : the Official Journal of the Japan Society of Hepatology
Y -J.Y. WanS W French

Abstract

Fatty acids are substrates and inducers for cytochrome P450 2E1 (CYP2E1) and peroxisome proliferator activated receptor alpha (PPARalpha). Previously, we have shown that the ethanol-induced CYP2E1 expression in rat is accompanied by the inhibition of the expression of the PPARalpha gene and the reduction in polyunsaturated fatty acid content. To further analyze the effect of CYP2E1 and ethanol in PPARalpha-mediated fatty acid homeostasis, the expression of PPARalpha and retinoid x receptor alpha (RXRalpha) and their target genes was examined in ethanol fed CYP2E1 deficient mice. Our data demonstrated that the expression of PPARalpha and RXRalpha genes was activated in the livers of CYP2E1-null mice suggesting a compensatory effect for the absence of CYP2El. In addition, the expression of PPARalpha target genes, which included the liver fatty acid-binding protein, malic enzyme, and CYP4A1 genes, was induced indicating the activation of PPARalpha-mediated pathways in CYP2E1 deficient mice. Ethanol inhibited the expression of some of the PPARalpha target genes in wild-type mouse livers, and the inhibitory effect of ethanol was particularly prominent in the CYP2E1-null mice. Morphologically, centrilobular fat accumulation was detec...Continue Reading

References

Oct 23, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·G RobertsonGeoffrey C Farrell
Apr 12, 2003·International Archives of Occupational and Environmental Health·Hermann M BoltRicarda Thier
Mar 23, 2004·The Journal of Biological Chemistry·Aparna VenkatramanShannon M Bailey
Jun 10, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Yuichi HaranoTakeshi Okanoue
Jun 3, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ying WangHelmut K Seitz
Jul 10, 2009·Archives of Toxicology·Tao Zeng, Ke-Qin Xie
Jan 3, 2018·International Journal of Molecular Sciences·Thaiz F BorinAli S Arbab
Jan 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Blair U BradfordIvan Rusyn
Apr 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yongke LuArthur I Cederbaum
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Emilia IpIsabelle Leclercq
Sep 9, 2006·Journal of Gastroenterology and Hepatology·David W Crabb, Suthat Liangpunsakul
Jun 8, 2011·International Journal of Experimental Pathology·Satoshi IkemuraYukihide Iwamoto

Related Concepts

Biochemical Pathway
Ethanol
PPARA wt Allele
Fatty Acid Homeostasis
Ethanol Measurement
Malate dehydrogenase (decarboxylating)
Fatty Liver
CYP2E1 gene
Retinoid X Receptor alpha
Cyp2e1

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