Abstract
Polyphosphoinositides are thought to be mediators of cellular signaling pathways as well as regulators of cytoskeletal elements and membrane trafficking events. It has recently been demonstrated that a class of phosphatidylinositol (PI) 3,4,5-P3 5'-phosphatases contains SH2 domains and proline-rich regions, which are present in many signaling proteins. We report here that insulin stimulation of Chinese hamster ovary cells (CHO-T) expressing human insulin receptors causes an 8-10-fold increase in PI 3,4,5-P3 5'-phosphatase activity in anti-phosphotyrosine immunoprecipitates of the cell lysates. This insulin-sensitive polyphosphoinositide 5'-phosphatase did not catalyze dephosphorylation of PI 4,5-P2. No change in 5'-phosphatase activity was detected in insulin receptor or IRS-1 immune complexes in response to insulin. However, insulin treatment of CHO-T cells markedly increased the PI 3,4,5-P3 5'-phosphatase activity associated with Shc and Grb2. The insulin-regulated polyphosphoinositide 5'-phosphatase was not immunoreactive with antibody raised against the recently cloned SHIP 5'-phosphatase reported to associate with Shc and Grb2 in B lymphocytes. These data demonstrate that insulin causes formation of complexes containing a ...Continue Reading
References
Nov 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G MyersM F White
Jul 4, 1991·Nature·X J SunM F White
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N B RudermanL C Cantley
Aug 17, 1990·European Journal of Biochemistry·S J MorganP J Parker
Sep 18, 1987·Science·O M Rosen
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y EbinaW J Rutter
Aug 15, 1970·Nature·U K Laemmli
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·O M RosenM H Cobb
Nov 24, 1995·The Journal of Biological Chemistry·T HarutaJ M Olefsky
Oct 1, 1995·Molecular and Cellular Biology·M J QuonS I Taylor
Aug 17, 1995·Nature·B M Burgering, P J Coffer
Apr 7, 1995·Science·Q HuL T Williams
Jan 1, 1995·The Biochemical Journal·P R ShepherdK Siddle
Oct 1, 1994·The Biochemical Journal·G I WelshC G Proud
Oct 1, 1994·The Biochemical Journal·D A CrossP Cohen
Jul 1, 1994·Molecular and Cellular Biology·B CheathamC R Kahn
Jul 1, 1994·Trends in Biochemical Sciences·M G MyersM F White
Aug 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Kapeller, L C Cantley
Mar 1, 1994·Molecular and Cellular Biology·G J PronkJ L Bos
Dec 29, 1995·The Journal of Biological Chemistry·R WoscholskiP J Parker
Apr 26, 1996·The Journal of Biological Chemistry·R A Heller-HarrisonM P Czech
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E DamenG Krystal
May 1, 1996·Genes & Development·M N LioubinL R Rohrschneider
Jun 7, 1996·The Journal of Biological Chemistry·J V VirbasiusM P Czech
Jul 26, 1996·The Journal of Biological Chemistry·S B WatersJ E Pessin
Jun 1, 1996·European Journal of Biochemistry·Y KabuyamaY Fukui
Apr 1, 1996·Current Biology : CB·W M KavanaughL T Williams
Dec 1, 1995·Current Biology : CB·L K MacDougallM D Waterfield
Citations
Jun 13, 2001·Biochemical and Biophysical Research Communications·D BleroC Erneux
Dec 5, 1998·Biochimica Et Biophysica Acta·C ErneuxX Pesesse
Nov 24, 1999·The Biochemical Journal·P B OateyJ M Tavaré
Feb 15, 2001·The Biochemical Journal·S StratfordS A Summers
Apr 10, 1999·Genes & Development·Q LiuJ M Penninger
Mar 10, 2001·Molecular and Cellular Biology·T WadaM Kobayashi
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z SongyangT F Franke
Sep 23, 1998·Experimental Cell Research·A M ValverdeM Lorenzo
Mar 7, 2006·Proteomics·Michał MikulaJerzy Ostrowski
Aug 29, 1998·The Journal of Biological Chemistry·A GuilhermeM P Czech
Dec 5, 1998·The Journal of Biological Chemistry·A Guilherme, M P Czech
Apr 25, 2000·The Journal of Biological Chemistry·N NakashimaJ M Olefsky
Feb 25, 2020·Current Topics in Medicinal Chemistry·Nne E UkoJ Phillip Bowen
Jun 5, 1998·The Journal of Biological Chemistry·T Maehama, J E Dixon
Jan 16, 1999·Molecular and Cellular Biology·P VollenweiderJ M Olefsky
Jun 21, 2007·The Journal of Biological Chemistry·Biao DongRihe Liu
Dec 29, 2018·The Journal of Cell Biology·Brady D GouldenGerald R V Hammond
Apr 27, 1999·FEBS Letters·A H LagrueM Jandrot-Perrus
Jun 4, 1998·Biochemical and Biophysical Research Communications·B C ShinK Takata
Jun 25, 1999·Biochemical and Biophysical Research Communications·H IshiharaM Kobayashi
Jun 2, 1998·Biochimica Et Biophysica Acta·T ShiraiY Fukui