PMID: 9168154Apr 26, 1997Paper

Regulation of phospholipase C delta1 by sphingosine

Biochimica Et Biophysica Acta
A Matecki, T Pawelczyk

Abstract

Sphingosine, which is on the pathway of sphingomyelin degradation, activates phospholipase C (PLC) delta1 moderately. In the liposome assay effect of sphingosine on PLC delta1 activity depends on KCl concentration. Stimulation of PLC delta1 by sphingosine increased as the KCl concentration is increased from 0 to 100 mM, and then diminished with the increasing KCl. In the liposome assay sphingosine diminishes inhibition of PLC delta1 by sphingomyelin. To determine the domain of PLC delta1 which interacts with sphingosine active proteolytic fragments of PLC delta1 were generated by trypsin digestion of the native enzyme. Sphingosine affects the activity of PLC delta1 fragment which lacked the amino-terminal domain (first 60 amino acids) but not the active fragment that has cleaved the domain spanning the X and Y region of PLC delta1. These observations indicate that for interaction of sphingosine with PLC delta1 intact domain that span regions of conservation, designated as X and Y is necessary. When the activity of PLC delta1 was assayed with PIP2 in the erythrocyte membrane as substrate, sphingosine strongly inhibited PLC delta1. The other homolog of sphingosine 4-hydroxysphinganine (phytosphingosine) inhibited PLC delta1 to mu...Continue Reading

References

Dec 1, 1989·The Biochemical Journal·P GascardF Giraud
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·R M BellY A Hannun
May 1, 1993·The Biochemical Journal·T Pawelczyk, J M Lowenstein

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Citations

Jul 19, 2002·Advances in Enzyme Regulation·Helene BirbesYusuf A Hannun
Aug 10, 2010·Journal of Colloid and Interface Science·Raina GeorgievaGalya Staneva
Mar 15, 2006·Biophysical Journal·F-Xabier ContrerasFélix M Goñi
Mar 16, 2018·Mediators of Inflammation·Sabine GröschElisabetta Albi
Feb 16, 2010·Molecular and Cellular Biochemistry·Lech Romanowicz, Edward Bańkowski
Jul 21, 2009·Clinical Biochemistry·Lech Romanowicz, Edward Bańkowski

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