Regulation of photomorphogenesis by expression of mammalian biliverdin reductase in transgenic Arabidopsis plants

The Plant Cell
D M LagariasJ C Lagarias

Abstract

The photoregulatory activity of the phytochrome photoreceptor requires the synthesis and covalent attachment of the linear tetrapyrrole prosthetic group phytochromobilin. Because the mammalian enzyme biliverdin IX alpha reductase (BVR) is able to functionally inactivate phytochromobilin in vitro, this investigation was undertaken to determine whether BVR expression in transgenic plants would prevent the synthesis of functionally active phytochrome in vivo. Here, we show that plastid-targeted, constitutive expression of BVR in Arabidopsis yields plants that display aberrant photomorphogenesis throughout their life cycle. Photobiological and biochemical analyses of three transgenic BVR lines exhibiting a 25-fold range of BVR expression established that the BVR-dependent phenotypes are light dependent, pleiotropic, and consonant with the loss of multiple phytochrome activities. Chlorophyll accumulation in BVR-expressing transgenic plants was particularly sensitive to increased light fluence rates, which is consistent with an important role for phytochrome in light tolerance. Under blue light, transgenic BVR plants displayed elongated hypocotyls but retained phototropic behavior and the ability to fully deetiolate. Directed BVR exp...Continue Reading

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Citations

Sep 20, 2005·Journal of Molecular Evolution·Patricia Lariguet, Christophe Dunand
Mar 6, 1999·Current Opinion in Plant Biology·B Grimm
Jan 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chitose KamiTakayuki Kohchi
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Takeshi KanegaeMasamitsu Wada
Jan 12, 2001·Plant Physiology·B L MontgomeryJ C Lagarias
Jun 13, 2001·Plant Physiology·M Santiago-OngE M Tobin
Jan 1, 1997·Annual Review of Cell and Developmental Biology·C Fankhauser, J Chory
Jun 3, 2010·Journal of Visualized Experiments : JoVE·Sankalpi N Warnasooriya, Beronda L Montgomery
Jan 1, 2004·The Arabidopsis Book·Haiyang Wang, Xing Wang Deng
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·S J DavisR D Vierstra
Nov 18, 2004·The Plant Journal : for Cell and Molecular Biology·Patricia Lariguet, Christian Fankhauser
Sep 13, 2003·The Plant Journal : for Cell and Molecular Biology·Keara A FranklinMatthew J Terry
May 13, 2006·The FEBS Journal·Janina HahnJon Hughes
Aug 26, 2009·Plant Signaling & Behavior·Beronda L Montgomery
Apr 2, 2011·Plant Signaling & Behavior·Sankalpi N WarnasooriyaBeronda L Montgomery
Feb 28, 1998·Current Biology : CB·J T Murphy, J C Lagarias
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Deqiang DuanmuJ Clark Lagarias
Mar 1, 2017·Plant, Cell & Environment·Deqiang DuanmuJ Clark Lagarias
Mar 30, 2001·The Journal of Biological Chemistry·C Fankhauser
Aug 22, 2013·Chemical Communications : Chem Comm·Konrad MüllerWilfried Weber

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