Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer

The Journal of Biological Chemistry
K S ChoiD M Waisman

Abstract

In a previous report we showed that plasmin-dependent lysis of a fibrin polymer, produced from purified components, was totally blocked if annexin II heterotetramer (AIIt) was present during fibrin polymer formation. Here, we show that AIIt inhibits fibrin clot lysis by stimulation of plasmin autodegradation, which results in a loss of plasmin activity. Furthermore, the C-terminal lysine residues of its p11 subunit play an essential role in the inhibition of fibrin clot lysis by AIIt. We also found that AIIt binds to fibrin with a K(d) of 436 nm and a stoichiometry of about 0.28 mol of AIIt/mol of fibrin monomer. The binding of AIIt to fibrin was not dependent on the C-terminal lysines of the p11 subunit. Furthermore, in the presence of plasminogen, the binding of AIIt to fibrin was increased to about 1.3 mol of AIIt/mol of fibrin monomer, suggesting that AIIt and plasminogen do not compete for identical sites on fibrin. Immunohistochemical identification of p36 and p11 subunits of AIIt in a pathological clot provides important evidence for its role as a physiological fibrinolytic regulator. These results suggest that AIIt may play a key role in the regulation of plasmin activity on the fibrin clot surface.

References

Nov 1, 1992·Cancer Metastasis Reviews·H C Kwaan
Sep 1, 1991·Progress in Cardiovascular Diseases·J HenkinH C Yang
Mar 1, 1991·Archives of Biochemistry and Biophysics·S S Husain
May 22, 1990·Biochemistry·N C KhannaD M Waisman
Jul 1, 1988·Seminars in Thrombosis and Hemostasis·P B Tracy
Nov 1, 1988·Annals of Emergency Medicine·D C Stump, K G Mann
Jun 1, 1995·Baillière's Clinical Haematology·H R Lijnen, D Collen
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E F PlowJ L Hoover-Plow
Mar 15, 1994·The Biochemical Journal·M JostV Gerke
Aug 1, 1995·Molecular and Cellular Biochemistry·D M Waisman
Nov 29, 1996·The Journal of Biological Chemistry·B NiliusG Droogmans
Jun 13, 1997·The Journal of Biological Chemistry·G KassamD M Waisman
Apr 15, 1997·Annals of the New York Academy of Sciences·K A Hajjar, J S Menell
Sep 1, 1995·Current Opinion in Hematology·K A Hajjar
Feb 21, 1998·Biochemistry·K S ChoiD M Waisman
Mar 21, 1998·The Journal of Biological Chemistry·G KassamD M Waisman
Dec 1, 1999·Trends in Cardiovascular Medicine·H M KangD M Waisman
Feb 2, 2000·Biochemistry·S L FitzpatrickD M Waisman
Mar 10, 2000·Biochimica Et Biophysica Acta·J MaiB F Sloane
Apr 25, 2000·The Journal of Biological Chemistry·J MaiB F Sloane
Nov 9, 2000·The Journal of Biological Chemistry·N R Filipenko, D M Waisman

❮ Previous
Next ❯

Citations

Mar 28, 2003·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Xiaohui ZhangShanjun Song
Jan 30, 2007·Breast Cancer Research : BCR·Gillian E StillfriedMarie Ranson
Feb 26, 2013·International Journal of Molecular Sciences·Patrícia A Madureira, David M Waisman
Mar 5, 2014·Disease Markers·Chi-Yun Wang, Chiou-Feng Lin
Feb 24, 2007·Journal of Neurochemistry·Wei-Qin Zhao, Bai Lu
Mar 29, 2005·The American Journal of Pathology·Djordje GvericM Louise Cuzner
Jun 27, 2006·Journal of Hepatology·Devanshi SethPaul S Haber
Feb 13, 2014·International Journal of Environmental Health Research·Brendan L MitchellBrian A Logue
Dec 12, 2003·The Journal of Biological Chemistry·Jennifer F CaplanDavid M Waisman
Aug 19, 2015·Cancer Growth and Metastasis·Seema Kumari, Ramarao Malla
Apr 26, 2005·The Journal of Biological Chemistry·Mijung KwonDavid M Waisman
Mar 21, 2018·Annals of Surgery·Lewis S GallUNKNOWN Targeted Action for Curing Trauma-Induced Coagulopathy (TACTIC) partners
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Mar 20, 2003·Microscopy Research and Technique·Rosario Donato
May 15, 2019·Annals of Surgery·Lewis S GallUNKNOWN Targeted Action for Curing Trauma-Induced Coagulopathy (TACTIC) partners
Nov 9, 2017·International Journal of Oncology·Maria V ChristensenEstrid V S Høgdall
Aug 4, 2020·Translational Vision Science & Technology·Rishika ChaudharyRichard J Blanch
Oct 1, 2003·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Arthur J Chu, Suresh T Mathews
Dec 22, 2011·Oncotarget·Patricia Alexandra MadureiraDavid Morton Waisman
Jan 29, 2021·The Journal of Clinical Endocrinology and Metabolism·Chia-Yi HsuEing-Mei Tsai

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

The Journal of Biological Chemistry
Nolan R FilipenkoD M Waisman
The Journal of Biological Chemistry
Mijung KwonD M Waisman
The Journal of Biological Chemistry
Nolan R Filipenko, D M Waisman
© 2021 Meta ULC. All rights reserved