Regulation of podocyte BK(Ca) channels by synaptopodin, Rho, and actin microfilaments.

American Journal of Physiology. Renal Physiology
Eun Young KimStuart E Dryer

Abstract

Mechanosensitive large-conductance Ca(2+)-activated K(+) channels encoded by the Slo1 gene (BK(Ca) channels) are expressed in podocytes. Here we show that BK(Ca) channels reciprocally coimmunoprecipitate with synaptopodin (Synpo) in mouse glomeruli, in mouse podocytes, and in a heterologous expression system (HEK293T cells) in which these proteins are transiently expressed. Synpo and Slo1 colocalize along the surface of the glomerular basement membrane in mouse glomeruli. Synpo interacts with BK(Ca) channels at COOH-terminal domains that overlap with an actin-binding domain on the channel molecule that is necessary for trafficking of BK(Ca) channels to the cell surface. Moreover, addition of exogenous beta-actin to mouse podocyte lysates reduces BK(Ca)-Synpo interactions. Coexpression of Synpo increases steady-state surface expression of BK(Ca) channels in HEK293T cells. However, Synpo does not affect the stability of cell surface BK(Ca) channels, suggesting a primary effect on the rate of forward trafficking, and Synpo coexpression does not affect BK(Ca) gating. Conversely, stable knockdown of Synpo expression in mouse podocyte cell lines reduces steady-state surface expression of BK(Ca) channels but does not affect total expr...Continue Reading

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Citations

Aug 6, 2010·American Journal of Physiology. Renal Physiology·Stuart E Dryer, Jochen Reiser
Oct 28, 2011·American Journal of Physiology. Renal Physiology·Eun Young KimStuart E Dryer
Jul 26, 2011·Journal of the American Society of Nephrology : JASN·John VassiliadisStefan Wawersik
Jun 21, 2014·Journal of Molecular Histology·Rolando Carrisoza-GaytánLaura I Escobar
Jun 11, 2011·Journal of Cellular Physiology·Eun Young Kim, Stuart E Dryer
Nov 13, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Pengjuan XuZhuo Yang
Dec 21, 2012·Human Molecular Genetics·Laurence MolinaBenjamin Delprat
Nov 5, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei YuFederico Sesti
Nov 8, 2012·Current Opinion in Nephrology and Hypertension·Michal Herman-EdelsteinUzi Gafter
May 10, 2013·American Journal of Physiology. Cell Physiology·Marc AndersonStuart E Dryer
Feb 3, 2018·International Journal of Molecular Medicine·Xiaocui ChenJu Liu
Dec 22, 2020·The Journal of Experimental Medicine·Shuai HuZhihong Liu
Jun 18, 2021·Small GTPases·Clara SteichenNicolas Bourmeyster

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