Regulation of polar morphogenesis in Caulobacter crescentus.

Journal of Bacteriology
A FukudaY Okada

Abstract

Deoxyribonucleic acid (DNA) phage phi CbK-resistant nonmotile mutants of Caulobacter crescentus CB15 were examined for their formation of polar surface structures (a stalk, a single flagellum, pili, and DNA phage receptors). These mutants were devoid of pili and DNA phage receptors and simultaneously defective either in both stalk formation and flagellar activity (stalk-defective type) or in the formation of normal flagella (flagella-defective type). DNA phage phi Cr30-mediated transductions revealed that stalk-defective mutants were of a single genetic type, whereas flagella-defective mutants were grouped into two different genetic types, I and II. To investigate how membrane proteins change in the above morphology mutants, cell envelopes pulse-labeled with L-[35S]methionine were analyzed by two-dimensional gel electrophoresis. No gross change of membrane proteins was observed in the stalk-defective mutant CB15 pdr-803, except a 49,000-molecular-weight (49K) protein which was found reduced. However, a 27K, two 28.5K, and a 70.5K protein were missing from the membrane of the flagella-defective type I mutant CB15 pdr-813. These proteins are most likely to be flagella-related protein, flagellins A and B, and hook protein, respect...Continue Reading

References

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Citations

Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Patrick D Curtis, Yves V Brun
Nov 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·J DeichW E Moerner
Aug 28, 2012·Journal of Virology·Gaël PanisPatrick H Viollier
May 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ricardo C Guerrero-FerreiraElizabeth R Wright
Jan 12, 2000·Journal of Bacteriology·M GoninY V Brun
Nov 26, 2015·Journal of Molecular Biology·Matthias ChristenBeat Christen
Mar 1, 1995·Microbiological Reviews·J W Gober, M V Marques
Apr 1, 1990·Journal of Bacteriology·A DriksB Ely
Dec 5, 1981·Journal of Molecular Biology·S KoyasuY Okada
Feb 15, 1984·Journal of Molecular Biology·S Koyasu, Y Shirakihara

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