PMID: 9531638Jun 6, 1998Paper

Regulation of poly(beta-hydroxybutyrate) synthesis in Methylobacterium rhodesianum MB 126 growing on methanol or fructose

Archives of Microbiology
G MothesW Babel

Abstract

The intracellular concentration of CoA metabolites and nucleotides was determined in batch cultures of Methylobacterium rhodesianum grown on methanol and shifted to growth on fructose. The intracellular concentration of CoA decreased from a high value of 0.6 nmol/mg poly(beta-hydroxybutyrate)-free bacterial dry mass during growth on methanol to a low value of 0.03 nmol/mg poly(beta-hydroxybutyrate)-free bacterial dry mass after a shift to fructose as a carbon source. The levels of NADH, NADPH, and acetyl-CoA were also lower. Under these conditions, acetyl-CoA was metabolized by both citrate synthase and beta-ketothiolase, and poly(beta-hydroxybutyrate) synthesis and growth occurred simultaneously during growth on fructose. Moreover, the level of ATP was approximately 50% lower during growth on fructose, supporting the hypothesis of a bottleneck in the energy supply during the growth of M. rhodesianum with fructose.

Citations

Jan 12, 2013·Applied Microbiology and Biotechnology·Kianoush Khosravi-DaraniKenji Tanaka
Aug 4, 2015·Journal of Biological Research·Paola Rivera-TercerosJorge Quillaguamán
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·L L Madison, G W Huisman

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