Regulation of potassium uptake in the sodium-resistant (NaCl(r)) and thalium-resistant (TlCl(r)) mutant strain of diazotrophic cyanobacterium Anabaena variabilis

Current Microbiology
Vinay S ChauhanP S Bisen

Abstract

A thalium chloride-resistant (TlCl(r)) mutant strain and a sodium chloride-resistant (NaCl(r)) mutant strain of the diazotrophic cyanobacterium Anabaena variabilis have been isolated by spontaneous and chemical mutagenesis by using TlCl, a potassium (K(+)) analog, and nitrosoguanidine (NTG), respectively. The TlCl(r) mutant strain was found to be defective in K(+) transport and showed resistance against 10 microM TlCl. However, it also showed sensitivity against NaCl (LD(50), 50 m M). In contrast, neither wild-type A. variabilis nor its NaCl(r) mutant strain could survive in the presence of 10 microM TlCl and died even at 1 microM TlCl. The TlCl(r) mutant strain exhibited almost negligible K(+) uptake, indicating the lack of a K(+) uptake system. High K(+) uptake was, however, observed in the NaCl(r) mutant strain, reflecting the presence of an active K(+) uptake system in this strain.DCMU, an inhibitor of PS II, inhibited the K(+) uptake in wild-type A. variabilis and its TlCl(r) and NaCl(r) mutant strains, suggesting that K(+) uptake in these strains is an energy-dependent process and that energy is derived from photophosphorylation. This contention is further supported by the inhibition of K(+) uptake under dark conditions. ...Continue Reading

Citations

Aug 31, 2014·Environmental Science and Pollution Research International·Heidi RenkemaEdward Berkelaar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.