PMID: 9654373Jul 8, 1998Paper

Regulation of promoter and intron enhancer activity in immunoglobulin heavy-chain genes during B-cell differentiation

Microbiology and Immunology
A NaitoT Azuma

Abstract

Chloramphenicol acetyltransferase (CAT) transgenic mice, in which the transgene is regulated by the VH promoter and heavy-chain intron enhancer (E mu), were examined to investigate the variation of activity of these cis-acting elements during the late stage of B-cell development. CAT enzyme activity decreased when resting B cells were stimulated through B-cell receptors (BCR) with goat anti-mouse IgM antibodies in vitro. On the other hand, when these B cells were stimulated by lipopolysaccharide (LPS) in vitro, they showed enhanced CAT activity, accompanied by an increase in the number of CD43+ B220+ cells (pro-plasma and plasma cells). In addition, the CAT activities in CD43+ B220+ and PNAhi B220+ cells from immunized mice were higher than those in CD43- B220+ and PNAlo B220+ cells, respectively. These results suggest that the activity of E mu in the context of VH promoter was transiently down-regulated by stimulation through the BCR but enhanced at the pro-plasma and plasma stages.

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Citations

May 7, 2013·Trends in Immunology·Guideng LiPaolo Casali
Jun 19, 2008·Immunology and Cell Biology·Anja GlanzBrigitte Biesinger

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