Regulation of prostacyclin and prostaglandin E(2) receptor mediated responses in adult rat dorsal root ganglion cells, in vitro

British Journal of Pharmacology
D K RowlandsH Wise

Abstract

1. Primary cultures of adult rat dorsal root ganglia (DRG) were prepared to examine the properties of prostacyclin (IP) receptors and prostaglandin E(2) (EP) receptors in sensory neurones. 2. IP receptor agonists, cicaprost and iloprost, stimulated adenylyl cyclase activity with EC(50) values of 22 and 28 nM, respectively. Prostaglandin E(1) (PGE(1)) and prostaglandin E(2) (PGE(2)) were 7 fold less potent than cicaprost and iloprost, with PGE(2) displaying a lower maximal response. 3. Adenylyl cyclase activation by iloprost, PGE(1) and PGE(2), but not by forskolin, was highly dependent on DRG cell density. Although the potency of iloprost and PGE(2) for stimulating adenylyl cyclase was unchanged, their maximal responses were significantly increased at low cell density. 4. Both IP and EP(2/4) receptors could be down-regulated by agonist pretreatment, however the presence of cyclo-oxygenase (COX) inhibitors did not prevent this apparent down-regulation of IP and EP(2/4) receptors at high DRG cell densities. 5. Stimulation of adenylyl cyclase by the neuropeptide calcitonin gene-related peptide was also decreased at high DRG cell density, whereas the responses to beta-adrenoceptor agonists were increased at high DRG cell density. 6...Continue Reading

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Citations

Jul 13, 2016·Journal of Neuroinflammation·Ramy Habashy MaltyMichael R Vasko
Aug 5, 2004·American Journal of Physiology. Renal Physiology·Ulla C KoppTomas Hökfelt
Aug 3, 2005·Hypertension·Vladislav SnitsarevFrancois M Abboud
May 26, 2005·Pharmacological Reviews·Vincenzo MollaceDaniela Salvemini
May 25, 2007·The Journal of Pharmacology and Experimental Therapeutics·Takuya NakayamaKenji Okajima

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