Regulation of prostaglandin E2 synthesis in human amnion by protein kinase C

Prostaglandins
J Sander, L Myatt

Abstract

A role for protein kinase C (PKC) in mediation of prostaglandin E2 synthesis in human amnion cells has been suggested. We have investigated the specificity of the stimulation of PGE2 synthesis by phorbol esters and employed putative PKC inhibitors to demonstrate the specificity of PKC stimulation. The three phorbol esters, tetradecanoyl phorbol-13-acetate, phorbol-12,13-dibutyrate and phorbol-12,13-didecanoate gave concentration-dependent (10(-10)-10(-7)M) increases in PGE2 synthesis when added to amnion cells in monolayer, however, no effect was seen with the structurally similar phorbols phorbol-12-13-diacetate, 4 alpha phorbol-12-13-didecanoate or phorbol base. The stimulatory effect of TPA (10(-8)M) on amnion PGE2 synthesis could be prevented by coincubation with the putative protein kinase C inhibitors 1-(5-isoquinoline sulphonyl) piperazine, 1-0-octadecyl-2-0-methyl-rac-glycero-3-phosphocholine, sphingosine and chlopromazine at concentrations of 10(-6)-10(-4)M. Addition of the transcription inhibitor actinomycin D at 10(-6)-10(-5)M prevented TPA (10(-8)M)-induced PGE2 synthesis. However, paradoxically, a further increase in PGE2 synthesis was seen when 10(-9)-10(-7)M actinomycin D was added together with TPA. The phosphol...Continue Reading

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Citations

Dec 1, 1992·Journal of Cellular Biochemistry·L MyattW V Everson
Jan 1, 1993·Calcified Tissue International·K M Reich, J A Frangos
Jun 12, 1993·Biochimica Et Biophysica Acta·M MizunumaH Kanoh
Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Leslie Myatt, Stephen J Lye
Aug 23, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Y KawanoI Miyakawa
Dec 1, 1990·American Journal of Obstetrics and Gynecology·D A KnissS G Gabbe
Jan 23, 1999·American Journal of Obstetrics and Gynecology·S D MooreL Myatt
Mar 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M D MitchellS S Edwin
Oct 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S EdwinM D Mitchell
Sep 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S S Edwin, M D Mitchell
Sep 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M D MitchellS Lundin-Schiller
Jan 24, 2008·Reproductive Sciences·Valentina CascianiJohn R G Challis
Oct 21, 2000·Endocrine Reviews· Challis JRGS J Lye

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