Regulation of protease-activated receptor-2 expression in gingival fibroblasts and Jurkat T cells by Porphyromonas gingivalis

Cell Biology International
Georgios N BelibasakisDurga Reddi

Abstract

Periodontal disease destroys the tooth-supporting tissues as a result of chronic inflammation elicited by bacterial accumulation on tooth surfaces. Porphyromonas gingivalis is a major periodontal pathogen, with a significant capacity to perturb connective tissue homeostasis and immune responses in the periodontium, attributed to its virulence factors, including a group of secreted cysteine proteases (gingipains). PAR-2 (protease-activated receptor-2) is a G-protein-coupled receptor activated upon proteolytic cleavage, mediating intracellular signalling events related to infection and inflammation, such as cytokine production. GF (gingival fibroblasts) and T cells have central roles in periodontal inflammation, which can potentially be mediated by PAR-2. The aims of this study were to investigate the effects of P. gingivalis on PAR-2 gene expression in human GF and Jurkat T cells, using quantitative real-time PCR, and to evaluate the involvement of gingipains. After 6 h of challenge with ascending concentrations of P. gingivalis, PAR-2 expression was up-regulated in both cell types by approximately 5-fold, compared with the control. The P. gingivalis concentration required for maximal PAR-2 induction was 4-fold greater in GF tha...Continue Reading

References

May 1, 1992·Journal of Periodontal Research·R L GregoryD R Lloyd
Apr 1, 1981·Infection and Immunity·J J ZambonJ Slots
Dec 11, 1997·Infection and Immunity·L KesavaluJ L Ebersole
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·R J Lamont, H F Jenkinson
Aug 12, 1999·Biochemical and Biophysical Research Communications·A BanbulaJ Potempa
Oct 16, 1999·Periodontology 2000·S C HoltC A Genco
Jan 5, 2000·Bone·L A AbrahamE J Mackie
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S SugawaraH Takada
Mar 10, 2001·Trends in Pharmacological Sciences·N VergnolleM D Hollenberg
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A Taubman, T Kawai
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·S FiorucciG Cirino
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akiko UeharaHaruhiko Takada
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akiko UeharaShunji Sugawara
Mar 27, 2004·Physiological Reviews·Valeria S Ossovskaya, Nigel W Bunnett
Aug 20, 2004·Current Medicinal Chemistry. Cardiovascular and Hematological Agents·Anne-Marie CoelhoNigel W Bunnett
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Whasun O ChungBeverly A Dale
Jan 26, 2005·Journal of Dental Research·M HolzhausenN Vergnolle
Apr 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Salunya TancharoenIkuro Maruyama
Mar 28, 2006·The American Journal of Pathology·Marinella HolzhausenNathalie Vergnolle
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rodrigo A GiacamanMark C Herzberg
Feb 26, 2009·Oral Microbiology and Immunology·N BostanciG N Belibasakis

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Citations

Apr 26, 2011·Journal of Periodontology·José Américo G FagundesMarinella Holzhausen
Feb 24, 2016·Frontiers in Microbiology·Kah Yan HowKok Gan Chan
Oct 2, 2014·BMC Microbiology·Kai BaoNagihan Bostanci
Aug 6, 2013·Journal of Clinical Periodontology·Asaf WilenskyLior Shapira
Apr 26, 2012·FEMS Microbiology Letters·Nagihan Bostanci, Georgios N Belibasakis
Apr 7, 2017·Molecular Oral Microbiology·Susanne BlochChristina Schäffer
Jan 26, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Eleonor PalmHazem Khalaf
Jul 4, 2017·Journal of Thrombosis and Haemostasis : JTH·B Isermann
Mar 7, 2020·Tissue Engineering. Part B, Reviews·Fahimeh TabatabaeiLobat Tayebi
Jan 12, 2020·Current Topics in Medicinal Chemistry·Abhishek WadhawanTeodor T Postolache

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