Regulation of protein A synthesis by the sar and agr loci of Staphylococcus aureus.

Infection and Immunity
A L CheungJ H Heinrichs

Abstract

The synthesis of protein A in Staphylococcus aureus is regulated by global regulatory loci such as sar and agr. Phenotypic data indicate that both sar and agr suppress protein A synthesis; like agr, sar also regulates protein A production at the transcriptional level. To determine the genetic requirement of sar in protein A suppression, we transformed shuttle plasmids containing various sar fragments into a sar mutant. Our results indicated that the 560-bp sarA transcript, or, more probably, the SarA protein (13.5 kDa), is sufficient for suppressing protein A gene transcription when introduced on a multicopy plasmid or as a single copy in the chromosome. Immunoblot analysis with a chicken anti-protein A antibody also confirmed the reduction in protein A expression in these sar mutant clones. Complementation studies revealed that the transcription of the protein A gene can be suppressed in a sar mutant background by a plasmid containing RNAIII. Surprisingly, in agr deletion mutant clones and in clones derived from the agr-sar double mutant, protein A gene transcription can also be suppressed by plasmids containing the sarA transcript plus additional upstream sequence but not the sarA transcript alone. These data suggest that the...Continue Reading

References

Jan 1, 1978·Journal of Immunological Methods·J W Goding
Mar 1, 1977·Infection and Immunity·P K PetersonP G Quie
Sep 25, 1972·European Journal of Biochemistry·I BjörkJ Sjöquist
Feb 1, 1994·Journal of Bacteriology·A L Cheung, P Ying
Nov 1, 1994·Analytical Biochemistry·A L CheungV A Fischetti
Jan 1, 1996·Journal of Bacteriology·J H HeinrichsA L Cheung
Aug 1, 1996·Journal of Bacteriology·M G BayerA L Cheung

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Citations

Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Jutta RossiBrigitte Berger-Bächi
Aug 23, 2003·Infection and Immunity·Katherine A SchmidtAmbrose L Cheung
Jan 2, 2004·Microbiology·Lindsey ShawSimon J Foster
May 20, 2006·The Journal of Biological Chemistry·Marisa I GómezAlice S Prince
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Greg A Somerville, Richard A Proctor
Sep 17, 2013·Biotechnology and Applied Biochemistry·Garshasb RigiKambiz Akbari Noghabi
Nov 5, 1998·Microbiology and Immunology·T OshidaT Matsushita
Dec 24, 2002·Infection and Immunity·Adhar C Manna, Ambrose L Cheung
Jun 4, 2004·Journal of Bacteriology·Jinxin Gao, George C Stewart
Apr 2, 2008·European Biophysics Journal : EBJ·Mathias SalomoFriedrich Kremer
Feb 19, 2015·American Journal of Respiratory Cell and Molecular Biology·Changsen WangWarren L Lee
Mar 26, 2003·Infection and Immunity·Costi D SifriStephen B Calderwood
Feb 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yingfang LiuGongyi Zhang
Jan 18, 2002·Infection and Immunity·Thanh LuongChia Y Lee
Feb 28, 1998·The Journal of Biological Chemistry·Y Chien, A L Cheung
Jul 3, 2003·Journal of Bacteriology·Ronggui LiGongyi Zhang
Feb 13, 2001·Infection and Immunity·A Manna, A L Cheung
Dec 3, 2008·Journal of Bacteriology·Nekane MerinoIñigo Lasa

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