Regulation of protein kinase C inactivation by Fas-associated protein with death domain.

The Journal of Biological Chemistry
Wei ChengZichun Hua

Abstract

Protein kinase C (PKC) plays important roles in diverse cellular processes. PKC has been implicated in regulating Fas-associated protein with death domain (FADD), an important adaptor protein involved in regulating death receptor-mediated apoptosis. FADD also plays an important role in non-apoptosis processes. The functional interaction of PKC and FADD in non-apoptotic processes has not been examined. In this study, we show that FADD is involved in maintaining the phosphorylation of the turn motif and hydrophobic motif in the activated conventional PKC (cPKC). A phosphoryl-mimicking mutation (S191D) in FADD (FADD-D) abolished the function of FADD in the facilitation of the turn motif and hydrophobic motif dephosphorylation of cPKC, suggesting that phosphorylation of Ser-191 negatively regulates FADD. We show that FADD interacts with PP2A, which is a major phosphatase involved in dephosphorylation of activated cPKC and FADD deficiency abolished PP2A mediated dephosphorylation of cPKC. We show that FADD deficiency leads to increased stability and activity of cPKC, which, in turn, promotes cytoskeleton reorganization, cell motility, and chemotaxis. Collectively, these results reveal a novel function of FADD in a non-apoptotic proc...Continue Reading

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Citations

Jan 30, 2015·Science Signaling·Brittany M BowmanStefanie Galbán
Aug 1, 2014·Biochemical and Biophysical Research Communications·Xiang-Yu ZhangZi-Chun Hua
Jul 1, 2016·EMBO Molecular Medicine·Hongqin ZhuangZi-Chun Hua
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Apr 30, 2017·Oncology Letters·Rong ZhangZi-Chun Hua
Dec 16, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Jiajia Li, Xianquan Zhan

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