Regulation of protein-protein binding by coupling between phosphorylation and intrinsic disorder: analysis of human protein complexes

Molecular BioSystems
Hafumi NishiAnna R Panchenko

Abstract

Phosphorylation offers a dynamic way to regulate protein activity, subcellular localization, and stability. The majority of signaling pathways involve an extensive set of protein-protein interactions, and phosphorylation is widely used to regulate protein-protein binding by affecting the stability, kinetics and specificity of interactions. Previously it was found that phosphorylation sites tend to be located on protein-protein binding interfaces and may orthosterically modulate the strength of interactions. Here we studied the effect of phosphorylation on protein binding in relation to intrinsic disorder for different types of human protein complexes with known structure of the binding interface. Our results suggest that the processes of phosphorylation, binding and disorder-order transitions are coupled to each other, with about one quarter of all disordered interface Ser/Thr/Tyr sites being phosphorylated. Namely, residue site disorder and interfacial states significantly affect the phosphorylation of serine and to a lesser extent of threonine. Tyrosine phosphorylation might not be directly associated with binding through disorder, and is often observed in ordered interface regions which are not predicted to be disordered in ...Continue Reading

References

Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·R W KriwackiP E Wright
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B A ShoemakerP G Wolynes
Nov 1, 2000·Cell·J Schlessinger
Dec 26, 2001·Chemical Reviews·L N Johnson, R J Lewis
Jan 5, 2002·The Journal of Biological Chemistry·Ying LiLaurence A Lasky
Sep 7, 2002·Journal of Molecular Biology·Jinfeng LiuBurkhard Rost
Oct 17, 2002·Journal of Molecular Biology·Lilia M IakouchevaA Keith Dunker
Feb 13, 2004·Nucleic Acids Research·Lilia M IakouchevaA Keith Dunker
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Feb 19, 2005·Current Opinion in Structural Biology·Anthony L Fink
Oct 13, 2005·The FEBS Journal·A Keith DunkerVladimir N Uversky
Apr 18, 2006·Structure·Shin-ichi TerawakiToshio Hakoshima
Apr 19, 2006·BMC Bioinformatics·Kang PengZoran Obradovic
Aug 29, 2006·Journal of Molecular Biology·Amrita MohanVladimir N Uversky
Sep 1, 2006·Molecular Biology of the Cell·Céline DerMardirossianGary M Bokoch
May 10, 2007·Journal of Proteome Research·Vladimir VacicA Keith Dunker
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Aug 8, 2007·Journal of Molecular Biology·Bálint MészárosZsuzsanna Dosztányi
Dec 25, 2007·Protein Science : a Publication of the Protein Society·Miho HigurashiKengo Kinoshita
Mar 28, 2008·Molecular Systems Biology·Philip M KimMark Gerstein
May 9, 2008·Molecular Systems Biology·Naoyuki SugiyamaYasushi Ishihama
Nov 11, 2008·Protein and Peptide Letters·Andrew CampenA Keith Dunker
Mar 14, 2009·PLoS Computational Biology·Jessica H FongAnna R Panchenko
May 5, 2009·PLoS Computational Biology·Bálint MészárosZsuzsanna Dosztányi
Jul 21, 2009·Biochemical Society Transactions·Louise N Johnson
Jan 26, 2010·Progress in Biophysics and Molecular Biology·Konstantin K TuroverovVladimir N Uversky
Jan 27, 2010·Biochimica Et Biophysica Acta·Bin XueVladimir N Uversky
Jun 15, 2010·Molecular BioSystems·Jessica H Fong, Anna R Panchenko
Jun 18, 2010·Journal of Bioinformatics and Computational Biology·Tatyana B MamonovaOxana V Galzitskaya
Nov 11, 2010·Protein Engineering, Design & Selection : PEDS·Yu XueJian Ren
Nov 11, 2010·Nucleic Acids Research·Holger DinkelFrancesca Diella
Nov 18, 2010·Nucleic Acids Research·Florian GnadMatthias Mann
Jul 23, 2011·Nature Reviews. Molecular Cell Biology·Rafael Garcia-MataKeith Burridge
Oct 21, 2011·Molecular BioSystems·Jessica H FongAnna R Panchenko
Dec 14, 2011·Structure·Hafumi NishiAnna R Panchenko

Citations

Dec 15, 2015·Current Opinion in Structural Biology·Anurag SethiMark Gerstein
Oct 3, 2015·Molecular Cell·Min ZhouDavid M Virshup
Dec 3, 2014·Journal of Molecular Biology·Hafumi NishiAnna R Panchenko
May 28, 2013·Current Opinion in Structural Biology·Marija BuljanM Madan Babu
Nov 11, 2015·Journal of Proteome Research·Mingming DongHanfa Zou
Aug 1, 2014·Progress in Biophysics and Molecular Biology·Govindarajan SudhaNarayanaswamy Srinivasan
Feb 18, 2014·Current Opinion in Structural Biology·Weijun Chen, Melissa J Moore
Jun 2, 2015·Frontiers in Plant Science·Slávka BekešováJozef Šamaj
Dec 17, 2014·Molecular Plant-microbe Interactions : MPMI·Stacy L DeBlasioMichelle Cilia
May 20, 2015·Frontiers in Molecular Biosciences·Lin LiEmil Alexov
Aug 20, 2014·Frontiers in Plant Science·R Shyama Prasad RaoJán A Miernyk
Aug 26, 2014·Frontiers in Genetics·Hafumi NishiAnna R Panchenko
Jan 27, 2015·Molecular & Cellular Proteomics : MCP·Rommel A MathiasIleana M Cristea
Mar 4, 2018·The Biochemical Journal·Kristen L HuberJeanne A Hardy
Aug 26, 2017·ELife·Adam Jm WollmanMark C Leake
May 7, 2014·The Journal of Biological Chemistry·Souvik ChakrabortyLinton M Traub
Oct 28, 2015·BMC Evolutionary Biology·Caitlyn VlasschaertDouglas A Gray
Jun 29, 2017·International Journal of Molecular Medicine·Fatima ArditoLorenzo Lo Muzio
Jan 22, 2020·Cellular and Molecular Life Sciences : CMLS·Françoise Schoentgen, Slavica Jonic
May 19, 2014·Intrinsically Disordered Proteins·Krishna D ReddyVladimir N Uversky
Dec 6, 2017·International Journal of Molecular Medicine·Zhengpeng YangDongbo Xue
Apr 1, 2013·Intrinsically Disordered Proteins·Bin Xue, Vladimir N Uversky
Apr 27, 2017·Biophysical Journal·Samuel LentonSusana C Marujo Teixeira
Mar 13, 2017·Journal of Molecular Biology·David J E CallawayZimei Bu
Jul 24, 2014·Journal of Cell Science·Priyanka GhonganeVictor M Bolanos-Garcia

Related Concepts

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.