Regulation of Proteolytic Enzyme Activity in Lactococcus lactis.

Applied and Environmental Microbiology
W MeijerJ Hugenholtz

Abstract

Two different Lactococcus lactis host strains, L. lactis subsp. lactis MG1363 and L. lactis subsp. cremoris SK1128, both containing plasmid pNZ521, which encodes the extracellular serine proteinase (PrtP) from strain SK110, were used to study the medium and growth-rate-dependent activity of three different enzymes involved in the proteolytic system of lactococci. The activity levels of PrtP and both the intracellular aminopeptidase PepN and the X-prolyl-dipeptidyl aminopeptidase PepXP were studied during batch and continuous cultivation. In both strains, the PrtP activity level was regulated by the peptide content of the medium. The highest activity level was found during growth in milk, and the lowest level was found during growth in the peptide-rich laboratory medium M17. Regulation of the intracellular peptidase activity appeared to be a strain-dependent phenomenon. In cells of strain MG1363, the activity levels of PepN and PepXP were regulated in a similar way to that observed for PrtP. In cells of strain SK1128, the levels of both peptidases were not significantly influenced by the peptide content of the medium. The presence of specific concentrations of the dipeptide prolylleucine could mimic the low activity levels of th...Continue Reading

References

Jan 1, 1992·Applied and Environmental Microbiology·P G BruinenbergW M De Vos
Jan 1, 1982·Methods in Enzymology·A J Hillier, G R Jago
Dec 1, 1984·Applied and Environmental Microbiology·J HugenholtzW N Konings
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Jan 1, 1992·Applied and Environmental Microbiology·P S TanW N Konings
Dec 1, 1993·Applied and Environmental Microbiology·P R Jensen, K Hammer

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Citations

Mar 7, 2001·Applied and Environmental Microbiology·S LuomaA Palva
Jul 16, 2003·Journal of Applied Microbiology·M PiuriS M Ruzal
Dec 22, 2015·Journal of Dairy Science·Tatsuro HagiMasaru Nomura
Nov 13, 1998·Journal of Bacteriology·G BuistJ Kok
Mar 21, 1998·Applied and Environmental Microbiology·A VerheulT Abee
Apr 4, 2000·Biotechnology and Bioengineering·M G WiebeA P Trinci
Jun 4, 2021·Probiotics and Antimicrobial Proteins·Kamel-Eddine El MecherfiColette Larré
Nov 6, 2004·International Journal of Food Microbiology·Yolanda SanzBert Poolman

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