Regulation of pulmonary venous tone in response to muscarinic receptor activation

American Journal of Physiology. Lung Cellular and Molecular Physiology
Xueqin Ding, Paul A Murray

Abstract

We investigated cellular mechanisms that mediate or modulate the vascular response to muscarinic receptor activation (ACh) in pulmonary veins (PV). Isometric tension was measured in isolated canine PV rings with endothelium (E+) and without endothelium (E-). Tension and intracellular Ca(2+) concentration ([Ca(2+)](i)) were measured simultaneously in fura-2-loaded E- PV strips. In the absence of preconstriction, ACh (0.01-10 microM) caused dose-dependent contraction in E+ and E- rings. ACh contraction was potentiated by removing the endothelium or by nitric oxide (NO) synthase inhibition (N-nitro-L-arginine methyl ester, P = 0.001). Cyclooxygenase inhibition (indomethacin) reduced ACh contraction in both E+ and E- PV rings (P = 0.013 and P = 0.037, respectively). ACh contraction was attenuated by inhibitors of voltage-operated Ca(2+) channels (nifedipine, P < 0.001), inositol-1,4,5-trisphosphate (IP(3))-mediated Ca(2+) release (2-aminoethoxydiphenyl borate, P = 0.001), PKC (bisindolylmaleimide I, P = 0.001), Rho-kinase (Y-27632, P = 0.002), and tyrosine kinase (TK; tyrphostin 47, P = 0.015) in E- PV rings. ACh (1 microM) caused a leftward shift in the [Ca(2+)](i)-tension relationship (P = 0.015), i.e., ACh increased myofilament ...Continue Reading

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Citations

Oct 3, 2006·European Journal of Pharmacology·Lynette FernandesRoy Goldie
Jun 21, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xueqin Ding, Paul A Murray
Mar 18, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mikyung YangPaul A Murray
May 13, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anne-Christine PeyterJean-François Tolsa
Feb 28, 2007·Anesthesiology·Xueqin Ding, Paul A Murray
Jul 10, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ciprian DospinescuStuart F Cruickshank

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