Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis.

Molecular Microbiology
Montira LeelakriangsakPeter Zuber

Abstract

Recently, we showed that the MarR-type repressor YkvE (MhqR) regulates multiple dioxygenases/glyoxalases, oxidoreductases and the azoreductase encoding yvaB (azoR2) gene in response to thiol-specific stress conditions, such as diamide, catechol and 2-methylhydroquinone (MHQ). Here we report on the regulation of the yocJ (azoR1) gene encoding another azoreductase by the novel DUF24/MarR-type repressor, YodB after exposure to thiol-reactive compounds. DNA binding activity of YodB is directly inhibited by thiol-reactive compounds in vitro. Mass spectrometry identified YodB-Cys-S-adducts that are formed upon exposure of YodB to MHQ and catechol in vitro. This confirms that catechol and MHQ are auto-oxidized to toxic ortho- and para-benzoquinones which act like diamide as thiol-reactive electrophiles. Mutational analyses further showed that the conserved Cys6 residue of YodB is required for optimal repression in vivo and in vitro while substitution of all three Cys residues of YodB affects induction of azoR1 transcription. Finally, phenotype analyses revealed that both azoreductases, AzoR1 and AzoR2 confer resistance to catechol, MHQ, 1,4-benzoquinone and diamide. Thus, both azoreductases that are controlled by different regulatory ...Continue Reading

References

May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Jan 1, 1992·Toxicology and Applied Pharmacology·T J MonksD G Graham
Jan 1, 1991·Chemico-biological Interactions·P J O'Brien
Sep 11, 1998·Archives of Microbiology·G P FergusonI R Booth
Jul 20, 1999·Chemical Research in Toxicology·G P FergusonY Al-Abed
Mar 1, 2000·Trends in Biochemical Sciences·M W Fraaije, A Mattevi
Apr 15, 2000·The Journal of Biological Chemistry·S BarbirzM O Glocker
Mar 12, 2002·FEMS Microbiology Letters·Yoshinari AndoKouji Nakamura
Apr 16, 2002·Chemical Research in Toxicology·Yoshito KumagaiNobuhiro Shimojo
May 23, 2002·Cell·Sung Oog KimJonathan S Stamler
Jul 9, 2002·Molecular Microbiology·Skorn Mongkolsuk, John D Helmann
Jul 26, 2002·Archives of Biochemistry and Biophysics·Henrikas NivinskasNarimantas Cenas
Mar 6, 2003·Journal of Bacteriology·Lars Ingo Ole LeichertMichael Hecker
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunji NakanoPeter Zuber
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunji NakanoPeter Zuber
Nov 18, 2003·Annual Review of Genetics·Mark S B Paget, Mark J Buttner
Dec 4, 2003·Biochemical Society Transactions·I R BoothS Kinghorn
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ivan Gusarov, Evgeny Nudler
Jul 20, 2006·Critical Reviews in Biochemistry and Molecular Biology·Frédéric H VaillancourtLindsay D Eltis
Dec 13, 2006·Science·Karen M Wassarman, Ruth M Saecker
Dec 13, 2006·Journal of Bacteriology·Montira Leelakriangsak, Peter Zuber
Feb 8, 2007·Bioscience, Biotechnology, and Biochemistry·Yoshiaki Nishiya, Yoshihiro Yamamoto
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin-Won LeeJohn D Helmann
Jul 6, 2007·The Journal of Biological Chemistry·Falko HochgräfeMichael Hecker

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Citations

Feb 25, 2009·Applied Microbiology and Biotechnology·Irina MugerfeldDorothea K Thompson
Jun 10, 2011·Applied Microbiology and Biotechnology·Sónia MendesLígia O Martins
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Quanjiang JiChuan He
Jul 16, 2010·Antioxidants & Redox Signaling·Haike Antelmann, John D Helmann
Jan 13, 2012·Nucleic Acids Research·Gottfried J PalmHaike Antelmann
Apr 14, 2009·Applied and Environmental Microbiology·Laura K JenningsJames M Gossett
Oct 20, 2009·Journal of Bacteriology·Dierk-Christoph PötherMichael Hecker
Jul 7, 2009·Annual Review of Microbiology·Peter Zuber
Feb 28, 2013·BMC Genomics·Jose Antonio IbarraLindsey N Shaw
Apr 27, 2010·Journal of Molecular Biology·Ali RyanEdith Sim
Aug 5, 2008·Molecular Microbiology·Manuel LiebekeHaike Antelmann
Aug 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sang Jae LeeBong-Jin Lee
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Jul 21, 2009·Biotechnology Journal·Pinar KocabaşTunçer H Ozdamar
Feb 20, 2008·Expert Review of Proteomics·Haike AntelmannPeter Zuber
Jul 17, 2019·Antioxidants & Redox Signaling·Verena Nadin FritschHaike Antelmann
Jul 21, 2010·The Journal of Biological Chemistry·Michael BussmannMichael Bott
Feb 27, 2015·Biological Chemistry·Melanie Hillion, Haike Antelmann
Jul 4, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sarah SainsburyRaymond J Owens
Aug 18, 2020·Applied and Environmental Microbiology·Luz I Valenzuela-GarcíaMario Pedraza-Reyes
Feb 6, 2021·Biological Chemistry·Nico LinznerHaike Antelmann
Apr 4, 2021·Antioxidants·In-Gyun Lee, Bong-Jin Lee
May 1, 2021·International Journal of Molecular Sciences·Karolina KoteckaAneta Agnieszka Bartosik
Jan 23, 2020·ACS Chemical Biology·Elvira RomeroNikola Lončar
Jun 8, 2021·Molecular Microbiology·Ankita J SachlaJohn D Helmann

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